Functional Reorganization of Visual Cortex Maps after Ischemic Lesions Is Accompanied by Changes in Expression of Cytoskeletal Proteins and NMDA and GABAA Receptor Subunits
Functional Reorganization of Visual Cortex Maps after Ischemic Lesions Is Accompanied by Changes in Expression of Cytoskeletal Proteins and NMDA and GABAA Receptor Subunits
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缺血性损伤后视觉皮层图谱的功能重组伴随着细胞骨架蛋白以及 NMDA 和 GABAA 受体亚基表达的变化
DOI：
10.1523/jneurosci.3213-03.2004
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
C. Arias
中科院分区：
文献类型：
--
作者：
A. Zepeda;F. Sengpiel;M. Guagnelli;L. Vaca;C. Arias
局灶性视觉皮层损伤后皮质表征的重组已被记录。有人提出，功能重组可能依赖于涉及兴奋性/抑制性神经传递平衡的改变的细胞机制以及病变周围神经元的形态变化。我们利用内在信号的光学成像探索了小猫初级视觉皮层局部缺血性病变后皮质视网膜专题图的功能重组。病灶后 1、2 和 5 周 (wPL)，我们探讨了功能重组是否与 MAP-2、GAP-43、GFAP、GABAA 受体亚基 α1 (GABAAα1)、NMDA 受体亚基 1 (NMDAR1) 的表达变化以及病灶边界的神经递质水平的变化及时相关。我们的结果表明：（1）视网膜专题图在缺血性病变后随着时间的推移而重新组织； (2) MAP-2水平从1wPL到5wPL逐渐增加； (3) MAP-2上调与病灶周围树突状结构的增加和GFAP阳性细胞的减少有关； (4) GAP-43水平在2wPL时达到最高点； (5) NMDAR1和谷氨酸含量从1wPL到5wPL平行增加； (6) GABAAα1水平从1wPL增加到2wPL，但在此时间点之后没有变化； (7)从1wPL到5wPL，GABA含量保持较低水平。这是一项综合研究，首次表明功能重组与树突萌芽以及先前提出参与皮质重塑的兴奋/抑制神经传递系统的变化及时相关，并提出了皮质表征可塑性可能发生的机制。
Reorganization of cortical representations after focal visual cortex lesions has been documented. It has been suggested that functional reorganization may rely on cellular mechanisms involving modifications in the excitatory/inhibitory neurotransmission balance and on morphological changes of neurons peripheral to the lesion. We explored functional reorganization of cortical retinotopic maps after a focal ischemic lesion in primary visual cortex of kittens using optical imaging of intrinsic signals. After 1, 2, and 5 weeks postlesion (wPL), we addressed whether functional reorganization correlated in time with changes in the expression of MAP-2, GAP-43, GFAP, GABAA receptor subunit α1 (GABAAα1), subunit 1 of the NMDA receptor (NMDAR1), and in neurotransmitter levels at the border of the lesion. Our results show that: (1) retinotopic maps reorganize with time after an ischemic lesion; (2) MAP-2 levels increase gradually from 1wPL to 5wPL; (3) MAP-2 upregulation is associated with an increase in dendritic-like structures surrounding the lesion and a decrease in GFAP-positive cells; (4) GAP-43 levels reach the highest point at 2wPL; (5) NMDAR1 and glutamate contents increase in parallel from 1wPL to 5wPL; (6) GABAAα1 levels increase from 1wPL to 2wPL but do not change after this time point; and (7) GABA contents remain low from 1wPL to 5wPL. This is a comprehensive study showing for the first time that functional reorganization correlates in time with dendritic sprouting and with changes in the excitatory/inhibitory neurotransmission systems previously proposed to participate in cortical remodeling and suggests mechanisms by which plasticity of cortical representations may occur.
影响因子：
3.7
作者：
JONES, EG
通讯作者：
JONES, EG
影响因子：
8.3
作者：
STROEMER, RP;KENT, TA;HULSEBOSCH, CE
通讯作者：
HULSEBOSCH, CE
影响因子：
8.3
作者：
Li, Y;Jiang, N;Chopp, M
通讯作者：
Chopp, M