Decreased sarcolipin protein expression and enhanced sarco(endo)plasmic reticulum Ca2+ uptake in human atrial fibrillation.
Decreased sarcolipin protein expression and enhanced sarco(endo)plasmic reticulum Ca2+ uptake in human atrial fibrillation.
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DOI：
10.1016/j.bbrc.2011.05.113
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发表时间：
2011-06-24
影响因子：
3.1
通讯作者：
Babu, Gopal J.
中科院分区：
文献类型：
--
作者：
Shanmugam, Mayilvahanan;Molina, Cristina E.;Gao, Shumin;Severac-Bastide, Renaud;Fischmeister, Rodolphe;Babu, Gopal J.
肌磷脂（Sarcolipin，SLN）是心肌肌浆网（SR）Ca ~（2+）ATP酶的关键调节因子，主要表达于心房，介导β-肾上腺素能反应。研究表明，SLN mRNA表达在人类慢性心房颤动（AF）和主动脉带状小鼠心房中降低;然而，SLN蛋白在人类心房病理中的表达及其在心房SR Ca 2+摄取中的作用尚未阐明。在本研究中，我们确定了主要的SR钙处理蛋白的表达在心房的人AF患者和人类和小鼠模型的心力衰竭（HF）。结果发现，压力超负荷性心力衰竭小鼠心房肌SR Ca ~（2+）摄取通道和Ca ~（2+）释放通道蛋白的表达明显降低，而心室肌SR Ca ~（2+）摄取通道和Ca ~（2+）释放通道蛋白的表达无明显变化。在人AF和HF中，SLN蛋白的表达显著降低，而其他主要SR Ca 2+处理蛋白的表达没有改变。此外，我们发现，SR Ca 2+摄取显着增加，在人类AF。选择性下调sarcolipin和增强SR Ca 2+摄取在人类AF表明，SLN下调可能发挥重要作用，在心房病理异常细胞内Ca 2+循环。
Sarcolipin (SLN), a key regulator of cardiac sarco(endo)plasmic reticulum (SR) Ca2+ ATPase, is predominantly expressed in atria and mediates β-adrenergic responses. Studies have shown that SLN mRNA expression is decreased in human chronic atrial fibrillation (AF) and in aortic banded mouse atria; however, SLN protein expression in human atrial pathology and its role in atrial SR Ca2+ uptake are not yet elucidated. In the present study, we determined the expression of major SR Ca2+ handling proteins in atria of human AF patients and in human and in a mouse model of heart failure (HF). We found that the expression of SR Ca2+ uptake and Ca2+ release channel proteins are significantly decreased in atria but not in the ventricles of pressure-overload induced HF in mice. In human AF and HF, the expression of SLN protein was significantly decreased; whereas the expressions of other major SR Ca2+ handling proteins were not altered. Further, we found that the SR Ca2+ uptake was significantly increased in human AF. The selective downregulation of sarcolipin and enhanced SR Ca2+ uptake in human AF suggest that SLN downregulation could play an important role in abnormal intracellular Ca2+ cycling in atrial pathology.
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N 末端的苏氨酸-5 可以调节心肌细胞中的肌磷脂功能。
影响因子：
5
作者：
Bhupathy P;Babu GJ;Ito M;Periasamy M
通讯作者：
Periasamy M
DOI：
10.1073/pnas.0707722104
发表时间：
2007-11-06
影响因子：
11.1
作者：
Babu, Gopal J.;Bhupathy, Poornima;Periasamy, Muthu
通讯作者：
Periasamy, Muthu
DOI：
10.1073/pnas.0402596101
发表时间：
2004-06-22
影响因子：
11.1
作者：
Asahi, M;Otsu, K;MacLennan, DH
通讯作者：
MacLennan, DH
影响因子：
10.8
作者：
Shanmugam, Mayilvahanan;Gao, Shumin;Babu, Gopal J.
通讯作者：
Babu, Gopal J.
DOI：
10.1111/j.1365-2362.2004.01422.x
发表时间：
2004-11-01
影响因子：
5.5
作者：
Uemura, N;Ohkusa, T;Ishikawa, Y
通讯作者：
Ishikawa, Y