Nucleolin regulates the proliferation of vascular smooth muscle cells in atherosclerotic via Aurora B.
Nucleolin regulates the proliferation of vascular smooth muscle cells in atherosclerotic via Aurora B.
复制标题
核素通过Aurora B调节动脉粥样硬化血管平滑肌细胞的增殖
DOI：
10.1111/jcmm.16125
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发表时间：
2021-01
影响因子：
5.3
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Sun H;Huang L;Liang P;Tang Y;Chen C;Chen H;Lin X;Luo Z;Li Y;Jiang B;Xiao X
血管平滑肌细胞（VSMC）在动脉粥样硬化中起重要作用。核仁素（nucleolin，NCL）是一种多功能蛋白质，参与许多重要的生理和病理过程。本研究旨在探讨核仁素在血管平滑肌细胞增殖和细胞周期中的作用。进展期斑块小鼠血管平滑肌细胞核仁素表达增加。在左颈总动脉结扎损伤模型中，免疫荧光染色显示，与右颈动脉相比，手术后小鼠左颈动脉中VSMCs的核仁素和Ki 67表达增加。POVPC或ox-LDL以剂量和时间依赖性方式上调HAVSMC中核仁素mRNA和蛋白表达。POVPC（5μg/ml）或ox-LDL（50μg/ml）促进HAVSMCs增殖。核仁素消融减轻了VSMC的促增殖作用。细胞周期分析和细胞能力结果显示，POVPC或ox-LDL促进HAVSMCs的增殖，而核仁素去除抑制HAVSMCs的增殖。核仁素缺失可阻止DNA复制于S期，使细胞周期阻滞于S期。生物信息学数据库预测蛋白质与核仁素和极光B的相互作用。核仁素过表达和消融影响极光B的表达。这些发现首次表明核仁素通过aurora B积极参与VSMCs的增殖。
Vascular smooth muscle cells (VSMCs) play a significant role in atherosclerosis. As a multifunctional protein, nucleolin (NCL) is involved in many important physiological and pathological processes. In this study, we aimed to investigate the role of nucleolin in VSMCs proliferation and cell cycle. The expression of nucleolin increased in VSMCs of mice with aortas advanced plaques. With the left common carotid‐artery ligation‐injury model, immunofluorescence staining revealed that nucleolin and Ki67 expression increased in VSMCs in mice left carotid artery compared with right carotid artery after surgery. POVPC or ox‐LDL up‐regulated nucleolin mRNA and protein expression in a dose‐ and time‐dependent manner in HAVSMCs. POVPC (5μg/ml) or ox‐LDL (50μg/ml) promoted the proliferation of HAVSMCs. Nucleolin ablation relieved the pro‐proliferation role of VSMCs. The cell cycle assay and cell ability results showing that POVPC or ox‐LDL increased the proliferation, but nucleolin ablation inhibited the proliferation of HAVSMCs. And nucleolin ablation can prevent DNA replication at S phase and induce cell cycle arrest in S phase. The bioinformatics database predicts protein‐protein interactions with nucleolin and aurora B. Nucleolin overexpression and ablation affected the expression of aurora B. These findings indicate for the first time that nucleolin actively involved the proliferation of VSMCs via aurora B.
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