The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain.
The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain.
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DOI：
10.1016/j.isci.2022.105425
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发表时间：
2022-11-18
期刊：
影响因子：
5.8
通讯作者：
Engl, Christoph
中科院分区：
文献类型：
--
作者：
Kotta-Loizou, Ioly;Giuliano, Maria Grazia;Jovanovic, Milija;Schaefer, Jorrit;Ye, Fuzhou;Zhang, Nan;Irakleidi, Danai Athina;Liu, Xiaojiao;Zhang, Xiaodong;Buck, Martin;Engl, Christoph
CRISPR相关Rossmann fold（CARF）结构域信号传导支持CRISPR-Cas核酸酶的调节;然而，RtcR CARF结构域控制两种保守RNA修复酶环化酶RtcA和连接酶RtcB的表达。在这里，我们证明了RtcAB是RtcR依赖性转录激活所需的，并直接结合到RtcR CARF。RtcAB催化活性对于与CARF的复合物形成不是必需的，但是对于RtcRAB依赖性转录激活是必需的，但不是充分的，这意味着需要额外的RNA修复依赖性激活信号。这种信号不同于寡腺苷酸，一种已知的CARF结构域配体，而是似乎起源于翻译装置：RtcB修复tmRNA，拯救停滞的核糖体并增加翻译延伸速度。总之，我们的数据提供了CARF结构域信号传导范围扩大的证据，包括其通过反式蛋白质-蛋白质相互作用控制的第一个证据，以及调节RNA修复的前馈机制，这是功能翻译装置所需的。RtcR通过其调节CARF结构域与RtcB和RtcA物理相互作用RtcR-RtcBA相互作用积极控制rtcBA表达转移信使RNA ssrA是rtc诱导应激的靶点RtcB影响翻译延伸的速度分子生物学;基因调控的分子机制;细胞生物学
CRISPR-associated Rossmann fold (CARF) domain signaling underpins modulation of CRISPR-Cas nucleases; however, the RtcR CARF domain controls expression of two conserved RNA repair enzymes, cyclase RtcA and ligase RtcB. Here, we demonstrate that RtcAB are required for RtcR-dependent transcription activation and directly bind to RtcR CARF. RtcAB catalytic activity is not required for complex formation with CARF, but is essential yet not sufficient for RtcRAB-dependent transcription activation, implying the need for an additional RNA repair-dependent activating signal. This signal differs from oligoadenylates, a known ligand of CARF domains, and instead appears to originate from the translation apparatus: RtcB repairs a tmRNA that rescues stalled ribosomes and increases translation elongation speed. Taken together, our data provide evidence for an expanded range for CARF domain signaling, including the first evidence of its control via in trans protein-protein interactions, and a feed-forward mechanism to regulate RNA repair required for a functioning translation apparatus. RtcR physically interacts with RtcB and RtcA via its regulatory CARF domain RtcR-RtcBA interactions positively control rtcBA expression The transfer-messenger RNA ssrA is a target of rtc inducing stress RtcB impacts the speed of translation elongation Molecular biology; Molecular mechanism of gene regulation; Cell biology
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影响因子：
5.2
作者：
Abo T;Chadani Y
通讯作者：
Chadani Y
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
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影响因子：
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作者：
Joly N;Burrows PC;Engl C;Jovanovic G;Buck M
通讯作者：
Buck M
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
16.8
作者：
Boël G;Smith PC;Ning W;Englander MT;Chen B;Hashem Y;Testa AJ;Fischer JJ;Wieden HJ;Frank J;Gonzalez RL Jr;Hunt JF
通讯作者：
Hunt JF