Cross-species, amplifiable microsatellite markers for neoverrucid barnacles from deep-sea hydrothermal vents developed using next-generation sequencing.
Cross-species, amplifiable microsatellite markers for neoverrucid barnacles from deep-sea hydrothermal vents developed using next-generation sequencing.
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DOI：
10.3390/ijms150814364
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发表时间：
2014-08-18
影响因子：
5.6
通讯作者：
Mitarai S
中科院分区：
文献类型：
--
作者：
Nakajima Y;Shinzato C;Khalturina M;Watanabe H;Inagaki F;Satoh N;Mitarai S
在西北太平洋深海热液喷口附近发现了大量的Neoverruca藤壶，对了解深海热液喷口动物群的种群形成和维持过程具有重要意义。利用新一代测序技术，我们从新疣藻中分离出12个多态性微卫星位点，在冲绳海槽收集的。每个微卫星位点显示2-19个等位基因。预期和观察到的杂合度范围分别为0.286至1.000和0.349至0.935。种间扩增结果表明，12个位点中有9个在马里亚纳海槽的短鳞新疣藻中成功扩增。使用9个位点计算的成对FST值表明两个物种之间存在显着的遗传分化。因此，我们开发的微卫星标记将有助于进一步的群体遗传学研究，以阐明遗传多样性，分化，分类和进化过程中的属Neoverruca。
Barnacles of the genus Neoverruca are abundant near deep-sea hydrothermal vents of the northwestern Pacific Ocean, and are useful for understanding processes of population formation and maintenance of deep-sea vent faunas. Using next-generation sequencing, we isolated 12 polymorphic microsatellite loci from Neoverruca sp., collected in the Okinawa Trough. These microsatellite loci revealed 2–19 alleles per locus. The expected and observed heterozygosities ranged from 0.286 to 1.000 and 0.349 to 0.935, respectively. Cross-species amplification showed that 9 of the 12 loci were successfully amplified for Neoverruca brachylepadoformis in the Mariana Trough. A pairwise FST value calculated using nine loci showed significant genetic differentiation between the two species. Consequently, the microsatellite markers we developed will be useful for further population genetic studies to elucidate genetic diversity, differentiation, classification, and evolutionary processes in the genus Neoverruca.
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DOI：
10.1111/j.1471-8286.2004.00684.x
发表时间：
2004-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Van Oosterhout, C;Hutchinson, WF;Shipley, P
通讯作者：
Shipley, P
影响因子：
2.5
作者：
Watanabe, H;Tsuchida, S;Kojima, S
通讯作者：
Kojima, S
影响因子：
7.7
作者：
Rousset, Francois
通讯作者：
Rousset, Francois
影响因子：
3.7
作者：
Castoe TA;Poole AW;de Koning AP;Jones KL;Tomback DF;Oyler-McCance SJ;Fike JA;Lance SL;Streicher JW;Smith EN;Pollock DD
通讯作者：
Pollock DD
影响因子：
5.8
作者：
Li, Weizhong;Godzik, Adam
通讯作者：
Godzik, Adam