Cardiac phosphoproteomics during remote ischemic preconditioning: a role for the sarcomeric Z-disk proteins.
Cardiac phosphoproteomics during remote ischemic preconditioning: a role for the sarcomeric Z-disk proteins.
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DOI：
10.1155/2014/767812
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发表时间：
2014
影响因子：
--
通讯作者：
Suleiman MS
中科院分区：
文献类型：
--
作者：
Abdul-Ghani S;Heesom KJ;Angelini GD;Suleiman MS
远端器官(如肢体)短暂的缺血/再灌注循环诱导的远端缺血预适应(RIPC)具有心脏保护作用。RIPC期间导致这种现象的心肌细胞变化目前尚不清楚。这项工作的目的是确定RIPC后通过磷酸化激活的心肌蛋白。为了实现我们的目标，我们使用了等压串联质量标记(TMT)和反相纳液色谱串联光谱，使用的是线性陷阱四极杆(LTQ)Orbitrap Velos质谱仪。雄性C57/BL6小鼠用三溴乙醇腹腔注射麻醉。在后肢周围放置一个袖带，并以200mmHg的压力充气，以防止血液流动，激光多普勒血流仪证实。肢体缺血5 m in，再灌流5 m in，共4个循环。提取心脏进行磷蛋白质组学研究。我们鉴定了大约30个响应RIPC方案的差异表达的磷酸蛋白。肌节Z盘中包括磷酸化肌粘蛋白-2在内的几种磷酸化蛋白的表达水平明显高于对照组。这项研究描述并验证了一种新的方法来监测心脏磷酸蛋白质组在RIPC心脏保护干预后和指数缺血之前的变化。磷酸化肌节蛋白水平的升高表明，它们可能在RIPC期间的心脏信号转导中发挥作用。
Remote ischemic preconditioning (RIPC) induced by brief ischemia/reperfusion cycles of remote organ (e.g., limb) is cardioprotective. The myocardial cellular changes during RIPC responsible for this phenomenon are not currently known. The aim of this work was to identify the activation by phosphorylation of cardiac proteins following RIPC. To achieve our aim we used isobaric tandem mass tagging (TMT) and reverse phase nanoliquid chromatography tandem spectrometry using a Linear Trap Quadropole (LTQ) Orbitrap Velos mass spectrometer. Male C57/Bl6 mice were anesthetized by an intraperitoneal injection of Tribromoethanol. A cuff was placed around the hind limb and inflated at 200 mmHg to prevent blood flow as confirmed by Laser Doppler Flowmetry. RIPC was induced by 4 cycles of 5 min of limb ischemia followed by 5 min of reperfusion. Hearts were extracted for phosphoproteomics. We identified approximately 30 phosphoproteins that were differentially expressed in response to RIPC protocol. The levels of several phosphoproteins in the Z-disk of the sarcomere including phospho-myozenin-2 were significantly higher than control. This study describes and validates a novel approach to monitor the changes in the cardiac phosphoproteome following the cardioprotective intervention of RIPC and prior to index ischemia. The increased level of phosphorylated sarcomeric proteins suggests they may have a role in cardiac signaling during RIPC.
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影响因子：
64.5
作者：
Huttlin EL;Jedrychowski MP;Elias JE;Goswami T;Rad R;Beausoleil SA;Villén J;Haas W;Sowa ME;Gygi SP
通讯作者：
Gygi SP
影响因子：
37.8
作者：
Hoole, Stephen P.;Heck, Patrick M.;Dutka, David P.
通讯作者：
Dutka, David P.
DOI：
10.4061/2009/239204
发表时间：
2009-12-08
期刊：
Human genomics and proteomics : HGP
影响因子：
--
作者：
Chandramouli, Kondethimmanahalli;Qian, Pei-Yuan
通讯作者：
Qian, Pei-Yuan
影响因子：
2.3
作者：
Ahmed, Rashed M.;Mohamed, El-Haddad A.;Mohamed, Tahir I.
通讯作者：
Mohamed, Tahir I.
影响因子：
20.1
作者：
GU, X;BISHOP, SP
通讯作者：
BISHOP, SP