γ-protocadherins are enriched and transported in specialized vesicles associated with the secretory pathway in neurons.
γ-protocadherins are enriched and transported in specialized vesicles associated with the secretory pathway in neurons.
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DOI：
10.1111/j.1460-9568.2010.07386.x
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发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
Phillips GR
中科院分区：
文献类型：
--
作者：
Fernández-Monreal M;Oung T;Hanson HH;O'Leary R;Janssen WG;Dolios G;Wang R;Phillips GR
γ原钙粘蛋白（Pcdh-γs）类似于经典钙粘蛋白，具有参与细胞间相互作用的潜力，具有嗜同性性质。新出现的证据表明，一些原钙粘蛋白在神经发育中的非传统作用。我们试图确定Pcdh-γ在神经元中的运输是否与这些分子的细胞内作用一致。在这里，我们表明，在经典的钙粘蛋白的大部分表面定位，内源性Pcdh-γs主要是在体内大鼠神经元的细胞内，平均分布在突触下树突和轴突室的细胞器。在出生后第16天，观察到突触隔室中含有Pcdh-γ的细胞器的比例显著较高。为了确定Pcdh-γ运输细胞器的来源，我们用Pcdh-γ抗体偶联的磁珠从脑细胞器悬浮液中分离细胞器。用Pcdh-γ抗体分离具有高水平COPII和内质网-高尔基体中间室（ERGIC）组分的囊泡，但用经典钙粘蛋白抗体不能分离。在培养的海马神经元中，Pcdh-γ免疫标记与树突中的calnexin和COPII阳性斑点部分重叠。通过实时成像，移动的Pcdh-γ-GFP谱与偶联到ER滞留信号的DsRed构建体动态共分布。Pcdh-γ的表达与树突中的管泡状细胞器和ER样细胞器的积累相关。我们的研究结果与Pcdh-γs可能在分泌途径中具有独特功能的可能性相一致，除了它们记录的表面作用。
Gamma protocadherins (Pcdh-γs) resemble classical cadherins and have the potential to engage in cell-cell interactions with homophilic properties. Emerging evidence suggests non-conventional roles for some protocadherins in neural development. We sought to determine if Pcdh-γ trafficking in neurons is consistent with an intracellular role for these molecules. Here we show that, in contrast to the largely surface localization of classical cadherins, endogenous Pcdh-γs are primarily intracellular in rat neurons in vivo and equally distributed within organelles of subsynaptic dendritic and axonal compartments. A strikingly higher proportion of Pcdh-γ-containing organelles in synaptic compartments was observed at post-natal day 16. To determine the origin of Pcdh-γ trafficking organelles, we isolated organelles with Pcdh-γ antibody coupled magnetic beads from brain organelle suspensions. Vesicles with high levels of COPII and endoplasmic reticulum-Golgi intermediate compartment (ERGIC) components were isolated with the Pcdh-γ antibody but not with the classical cadherin antibody. In cultured hippocampal neurons, Pcdh-γ immunolabeling partially overlapped with calnexin and COPII- positive puncta in dendrites. Mobile Pcdh-γ-GFP profiles dynamically codistributed with a DsRed construct coupled to ER retention signals by live imaging. Pcdh-γ expression correlated with accumulations of tubulovesicular and ER-like organelles in dendrites. Our results are consistent with the possibility that Pcdh-γs could have a unique function with the secretory pathway in addition to their documented surface roles.
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影响因子：
7.8
作者：
Fu, LW;Sztul, E
通讯作者：
Sztul, E
DOI：
10.1083/jcb.200802069
发表时间：
2008-07-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nakao S;Platek A;Hirano S;Takeichi M
通讯作者：
Takeichi M
影响因子：
16.2
作者：
Fannon, AM;Colman, DR
通讯作者：
Colman, DR
影响因子：
5.3
作者：
Aridor, M;Guzik, AK;Fish, KN
通讯作者：
Fish, KN
影响因子：
4.8
作者：
Morishita, Hirofumi;Umitsu, Masataka;Ikegami, Takahisa
通讯作者：
Ikegami, Takahisa