Kidney epithelial cells are active mechano-biological fluid pumps.
Kidney epithelial cells are active mechano-biological fluid pumps.
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DOI：
10.1038/s41467-022-29988-w
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发表时间：
2022-04-28
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
The role of mechanical forces driving kidney epithelial fluid transport and morphogenesis in kidney diseases is unclear. Here, using a microfluidic platform to recapitulate fluid transport activity of kidney cells, we report that renal epithelial cells can actively generate hydraulic pressure gradients across the epithelium. The fluidic flux declines with increasing hydraulic pressure until a stall pressure, in a manner similar to mechanical fluid pumps. For normal human kidney cells, the fluidic flux is from apical to basal, and the pressure is higher on the basal side. For human Autosomal Dominant Polycystic Kidney Disease cells, the fluidic flux is reversed from basal to apical. Molecular and proteomic studies reveal that renal epithelial cells are sensitive to hydraulic pressure gradients, changing gene expression profiles and spatial arrangements of ion exchangers and the cytoskeleton in different pressure conditions. These results implicate mechanical force and hydraulic pressure as important variables during kidney function and morphological change, and provide insights into pathophysiological mechanisms underlying the development and transduction of hydraulic pressure gradients. How mechanical forces drive fluid transport in the kidney remains unclear. Here, the authors use a microfluidic platform to show that kidney epithelial cells generate hydraulic pressure gradients across the epithelium, and that the fluid flux is from apical to basal for normal cells, and inverted in autosomal dominant polycystic kidney disease cells.
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DOI：
10.1056/nejmoa1205511
发表时间：
2012-12-20
期刊：
The New England journal of medicine
影响因子：
--
作者：
Torres VE;Chapman AB;Devuyst O;Gansevoort RT;Grantham JJ;Higashihara E;Perrone RD;Krasa HB;Ouyang J;Czerwiec FS;TEMPO 3:4 Trial Investigators
通讯作者：
TEMPO 3:4 Trial Investigators
水渗透驱动肿瘤细胞在受限微环境中迁移
影响因子：
64.5
作者：
Stroka KM;Jiang H;Chen SH;Tong Z;Wirtz D;Sun SX;Konstantopoulos K
通讯作者：
Konstantopoulos K
影响因子：
4
作者：
Dixon, Eryn E.;Maxim, Demetrios S.;Woodward, Owen M.
通讯作者：
Woodward, Owen M.
影响因子：
64.8
作者：
Gudipaty SA;Lindblom J;Loftus PD;Redd MJ;Edes K;Davey CF;Krishnegowda V;Rosenblatt J
通讯作者：
Rosenblatt J
DOI：
10.1083/jcb.201006173
发表时间：
2010-11-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chapin HC;Caplan MJ
通讯作者：
Caplan MJ