A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringae pathovar tabaci 11528.
A draft genome sequence and functional screen reveals the repertoire of type III secreted proteins of Pseudomonas syringae pathovar tabaci 11528.
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DOI：
10.1186/1471-2164-10-395
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发表时间：
2009-08-24
期刊：
影响因子：
4.4
通讯作者：
Rathjen JP
中科院分区：
文献类型：
--
作者：
Studholme DJ;Ibanez SG;MacLean D;Dangl JL;Chang JH;Rathjen JP
假单胞菌是一种广泛分布的细菌病原体，其在广泛的经济上重要的植物物种上引起疾病。P.erythingae菌株的致病性依赖于III型分泌系统，其将一套多达约30种毒力“效应”蛋白分泌到宿主细胞质中，在那里它们破坏真核细胞生理学并破坏宿主防御。烟草疫霉致病变种（P.lagingae pathovar tabaci）自然地在野生烟草上引起疾病，野生烟草是茄科（Solanaceae）的模式成员，茄科包括许多作物物种以及大豆。我们使用“下一代”Illumina测序平台和Velvet短读组装程序来生成烟草疫霉致病变种菌株11528的145 X深6，077，921个核苷酸的基因组草图序列。根据我们的组装草案，我们预测了5，300个编码至少100个氨基酸长的蛋白质的潜在基因，其中303个（5.72%）与之前完全测序的三个P. erythingae基因组编码的蛋白质没有显著的序列相似性。在三种先前完全测序的P.eriningae菌株中保守的核心Hrp外部蛋白组中，大多数在菌株11528中也是保守的，包括AvrE1、HopAH2、HopAJ2、HopAK1、HopAN1、HopI、HopJ1、HopX1、HrpK1和HrpW1。然而，在11528中hrpZ1基因部分缺失，hopAF 1完全缺失。菌株11528的基因组草图还编码HopO 1、HopT 1、HopAH 1、HopR 1、HopV 1、HopAG 1、HopAS 1、HopAE 1、HopAR 1、HopF 1和HopW 1的紧密同源物以及简并HopM 1 ′。利用功能筛选，我们证实hopO1、hopT1、hopAH1、hopM1 '、hopAE1、hopAR1和hopAI1'是与毒性相关的HrpL调节子的一部分，尽管hopAI1'和hopM1'序列是简并的，具有过早的终止密码子。我们还发现了两个额外的HrpL调节的效应候选人和一个HrpL调节的avrPto1的远端同源物。基因组序列草图有助于继续开发野生烟草上的烟草疫霉作为研究植物细菌性疾病的有吸引力的模型系统。这些效应子不仅为植物防御机制的研究提供了一套分子工具，而且为致病性和寄主特异性的进化提供了进一步的线索。我们还发现了几个大的基因组区域在Pta 11528不共享可检测的核苷酸序列相似性与以前测序的假单胞菌基因组。这些地区可能包括水平收购的岛屿，可能有助于致病性或附生健身的Pta 11528。
Pseudomonas syringae is a widespread bacterial pathogen that causes disease on a broad range of economically important plant species. Pathogenicity of P. syringae strains is dependent on the type III secretion system, which secretes a suite of up to about thirty virulence 'effector' proteins into the host cytoplasm where they subvert the eukaryotic cell physiology and disrupt host defences. P. syringae pathovar tabaci naturally causes disease on wild tobacco, the model member of the Solanaceae, a family that includes many crop species as well as on soybean. We used the 'next-generation' Illumina sequencing platform and the Velvet short-read assembly program to generate a 145X deep 6,077,921 nucleotide draft genome sequence for P. syringae pathovar tabaci strain 11528. From our draft assembly, we predicted 5,300 potential genes encoding proteins of at least 100 amino acids long, of which 303 (5.72%) had no significant sequence similarity to those encoded by the three previously fully sequenced P. syringae genomes. Of the core set of Hrp Outer Proteins that are conserved in three previously fully sequenced P. syringae strains, most were also conserved in strain 11528, including AvrE1, HopAH2, HopAJ2, HopAK1, HopAN1, HopI, HopJ1, HopX1, HrpK1 and HrpW1. However, the hrpZ1 gene is partially deleted and hopAF1 is completely absent in 11528. The draft genome of strain 11528 also encodes close homologues of HopO1, HopT1, HopAH1, HopR1, HopV1, HopAG1, HopAS1, HopAE1, HopAR1, HopF1, and HopW1 and a degenerate HopM1'. Using a functional screen, we confirmed that hopO1, hopT1, hopAH1, hopM1', hopAE1, hopAR1, and hopAI1' are part of the virulence-associated HrpL regulon, though the hopAI1' and hopM1' sequences were degenerate with premature stop codons. We also discovered two additional HrpL-regulated effector candidates and an HrpL-regulated distant homologue of avrPto1. The draft genome sequence facilitates the continued development of P. syringae pathovar tabaci on wild tobacco as an attractive model system for studying bacterial disease on plants. The catalogue of effectors sheds further light on the evolution of pathogenicity and host-specificity as well as providing a set of molecular tools for the study of plant defence mechanisms. We also discovered several large genomic regions in Pta 11528 that do not share detectable nucleotide sequence similarity with previously sequenced Pseudomonas genomes. These regions may include horizontally acquired islands that possibly contribute to pathogenicity or epiphytic fitness of Pta 11528.
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影响因子：
4.4
作者：
Aury JM;Cruaud C;Barbe V;Rogier O;Mangenot S;Samson G;Poulain J;Anthouard V;Scarpelli C;Artiguenave F;Wincker P
通讯作者：
Wincker P
影响因子：
7
作者：
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通讯作者：
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影响因子：
2.1
作者：
Farrer, Rhys A.;Kemen, Eric;Studholme, David J.
通讯作者：
Studholme, David J.
DOI：
10.1073/pnas.0409660102
发表时间：
2005-02-15
影响因子：
11.1
作者：
Chang, JH;Urbach, JM;Dangl, JL
通讯作者：
Dangl, JL
DOI：
10.1073/pnas.1731982100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Buell, CR;Joardar, V;Collmer, A
通讯作者：
Collmer, A