Arthritis imaging using a near-infrared fluorescence folate-targeted probe.
Arthritis imaging using a near-infrared fluorescence folate-targeted probe.
复制标题
通过近红外荧光叶酸靶向探针进行关节炎成像。
DOI：
10.1186/ar1483
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发表时间：
2005
影响因子：
4.9
通讯作者：
Tung, CH
中科院分区：
文献类型：
--
作者：
Chen, WT;Mahmood, U;Weissleder, R;Tung, CH
最近开发的近红外荧光标记的叶酸探针（NIR 2-叶酸）进行了测试，在体内成像关节炎使用脂多糖关节内注射模型和KRN转基因小鼠血清诱导小鼠模型。在脂多糖注射模型中，在脂多糖处理的关节和对照关节之间比较NIR 2-叶酸（n = 12）和游离NIR 2（n = 5）的荧光信号强度。发现炎症关节处NIR 2-叶酸探针的荧光信号强度显著高于对照正常关节（高达2.3倍，P < 0.001）。无NIR 2染料注射组显示出比NIR 2-叶酸探针注射组持续更低的增强比率。还给予过量的叶酸以证明与NIR 2-叶酸的竞争作用。在KRN血清转移模型（n = 4）中，在血清转移后的不同时间点应用NIR 2-叶酸，并且早在致关节炎抗体转移后30小时就可以检测到发炎的关节（信号强度增加1.8倍）。荧光显微镜、组织学和免疫组织化学验证了光学成像结果。我们的结论是，在体内关节炎检测是可行的，使用叶酸靶向近红外荧光探针。这种受体靶向成像方法可以通过提供炎性关节疾病中活性巨噬细胞状态的体内表征来促进改善关节炎诊断和疾病进展的早期评估。
A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence signal intensity of NIR2-folate (n = 12) and of free NIR2 (n = 5) was compared between lipopolysaccharide-treated and control joints. The fluorescence signal intensity of the NIR2-folate probe at the inflammatory joints was found to be significantly higher than the control normal joints (up to 2.3-fold, P < 0.001). The NIR2-free dye injection group showed a persistent lower enhancement ratio than the NIR2-folate probe injection group. Excessive folic acid was also given to demonstrate a competitive effect with the NIR2-folate. In the KRN serum transfer model (n = 4), NIR2-folate was applied at different time points after serum transfer, and the inflamed joints could be detected as early as 30 hours after arthritogenic antibody transfer (1.8-fold increase in signal intensity). Fluorescence microscopy, histology, and immunohistochemistry validated the optical imaging results. We conclude that in vivo arthritis detection was feasible using a folate-targeted near-infrared fluorescence probe. This receptor-targeted imaging method may facilitate improved arthritis diagnosis and early assessment of the disease progress by providing an in vivo characterization of active macrophage status in inflammatory joint diseases.
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