CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin.
CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin.
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CD44 交联通过上调 p-Moesin 增加乳腺癌的恶性程度
DOI：
10.1186/s12935-020-01663-4
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发表时间：
2020-11-23
影响因子：
5.8
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Hu S;Shi X;Liu Y;He Y;Du Y;Zhang G;Yang C;Gao F
研究背景CD 44在大多数肿瘤细胞中高表达，其交联模式与肿瘤的迁移和侵袭密切相关。然而，在癌细胞转移过程中关于CD 44交联的潜在分子机制知之甚少。因此，本研究的目的是探讨是否破坏CD 44交联在乳腺癌细胞中可以阻止细胞的迁移和侵袭，并确定CD 44交联对癌细胞的恶性程度的影响。方法CD 44，CD 44交联和Moesin磷酸化在乳腺癌细胞中的表达通过Western Blot检测。Transwell法检测CD 44交联对肿瘤转移的影响。使用CCK-8测定评估CD 44交联破坏对细胞活力的影响。结果CD 44交联蛋白在浸润性乳腺癌细胞（BT-549和MDA-MB-231）中高表达，与乳腺癌的恶性程度有关。一组乳腺癌细胞系中ERM复合物的表达表明Moesin及其磷酸化可能在细胞转移中起重要作用。Moesin磷酸化被乳腺癌细胞中的CD 44去交联抑制，Moesin shRNA敲低减弱了CD 44交联对细胞迁移和侵袭的促进作用。最后，免疫组化结果表明，p-Moesin过表达在原发性和转移cancers.ConclusionsOur研究表明，CD 44交联可以提高p-Moesin的表达，并进一步影响迁移和乳腺癌细胞的侵袭。这些结果也表明，p-Moesin可能在未来的靶向癌症治疗中是有用的。
BackgroundCD44 is highly expressed in most cancer cells and its cross-linking pattern is closely related to tumor migration and invasion. However, the underlying molecular mechanism regarding CD44 cross-linking during cancer cell metastasis is poorly understood. Therefore, the purpose of this study was to explore whether disruption of CD44 cross-linking in breast cancer cells could prevent the cells migration and invasion and determine the effects of CD44 cross-linking on the malignancy of the cancer cells.MethodsThe expression of CD44, CD44 cross-linking and Moesin phosphorylation in breast cancer cells was assessed by Western Blot assays. Effects of CD44 cross-linking on tumor metastasis were evaluated by Transwell assay. The effects of CD44 cross-linking disruption on cell viability were assessed using CCK-8 assays. The expression of p-Moesin between normal and breast cancer tissues was examined by immunohistochemical staining.ResultsHigh expression of CD44 cross-linking was found in invasive breast cancer cells (BT-549 and MDA-MB-231), which is associated with the malignancy of breast cancer. The expressions of ERM complex in a panel of breast cancer cell lines indicate that Moesin and its phosphorylation may play a significant role in cell metastasis. Moesin phosphorylation was inhibited by CD44 de-crosslinking in breast cancer cells and Moesin shRNA knockdown attenuated the promotion of CD44 cross-linking on cell migration and invasion. Finally, immunohistochemistry results demonstrated that p-Moesin was overexpressed in primary and metastatic cancers.ConclusionsOur study suggested that CD44 cross-linking could elevate p-Moesin expression and further affect migration and invasion of breast cancer cells. These results also indicate that p-Moesin may be useful in future targeted cancer therapy.
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DOI：
10.1083/jcb.135.4.1139
发表时间：
1996-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sleeman J;Rudy W;Hofmann M;Moll J;Herrlich P;Ponta H
通讯作者：
Ponta H
影响因子：
28.2
作者：
Liu X;Taftaf R;Kawaguchi M;Chang YF;Chen W;Entenberg D;Zhang Y;Gerratana L;Huang S;Patel DB;Tsui E;Adorno-Cruz V;Chirieleison SM;Cao Y;Harney AS;Patel S;Patsialou A;Shen Y;Avril S;Gilmore HL;Lathia JD;Abbott DW;Cristofanilli M;Condeelis JS;Liu H
通讯作者：
Liu H
影响因子：
6.9
作者：
Karousou, Evgenia;Misra, Suniti;Skandalis, Spyros S.
通讯作者：
Skandalis, Spyros S.
DOI：
10.1073/pnas.0703478104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Dalerba, Piero;Dylla, Scott J.;Clarke, Michael F.
通讯作者：
Clarke, Michael F.
LYVE-1 与细胞表面透明质酸之间的相互作用可能在癌细胞粘附的多样性中发挥作用
影响因子：
3.7
作者：
Du, Yan;Liu, Hua;Gao, Feng
通讯作者：
Gao, Feng