Transformed mouse glucocorticoid receptor: generation and interconversion of the 3.8S, monomeric and 5.2S, oligomeric species.
Transformed mouse glucocorticoid receptor: generation and interconversion of the 3.8S, monomeric and 5.2S, oligomeric species.
复制标题
转化的小鼠糖皮质激素受体：3.8S单体和5.2S寡聚体的产生和相互转化。
作者：
C. E. Reker;B. Kovacic;S. B. Eastman‐Reks;W. Vedeckis
最近的研究表明亚基解离是糖皮质激素受体转化的可能机制[Vedeckis，W. V.（1983）Biochemistry 22，1983-1989; Raaka，B.M.，& Samuels，H.H.（1983）J.Biol.Chem.258，417-425]。尽管越来越明显的是，来自小鼠AtT-20细胞的未转化（非核结合和非DNA结合）糖皮质激素受体是9.1S寡聚物种类（Mr 290 000-360 000），但已描述了该受体的两种转化种类。其中一个亚基的沉降系数为5.2 S（在含酒石酸盐的梯度上），而最小的非蛋白水解单体亚基为3.8 S。本研究的目的是确定哪一种是在体外和体内产生的最常见的形式，以及这两种形式之间的结构关系。各种各样的体外转化方案都产生了5.2S的形式时，通过使用一个垂直管转子的含蔗糖梯度的双甘酯进行分析。动力学研究表明，5.2S形式的出现正好与转化受体的出现相吻合，如通过DEAE-纤维素洗脱所定义的。此外，当直接从蔗糖梯度收集3.8S和5.2S峰时，它们是转化的受体，如通过DEAE-纤维素和DNA-纤维素色谱所定义的，而当使用相同的标准时，9.1S蔗糖梯度峰是未转化的。当从高盐蔗糖梯度中分离出3.8S单体，然后脱盐时，3.8S单体恢复为5.2S形式（含酒石酸盐梯度）或6.6S形式（低盐、无酒石酸盐梯度）。（250字处删节）
Recent studies have implicated subunit dissociation as a possible mechanism of glucocorticoid receptor transformation [Vedeckis, W.V. (1983) Biochemistry 22, 1983-1989; Raaka, B.M., & Samuels, H.H. (1983) J. Biol. Chem. 258, 417-425]. While it is becoming increasingly evident that the untransformed (non-nuclear-binding and non-DNA-binding) glucocorticoid receptor from mouse AtT-20 cells is a 9.1S oligomeric species (Mr 290 000-360 000), two transformed species have been described for this receptor. One of these has a sedimentation coefficient of 5.2 S (on molybdate-containing gradients), while the smallest nonproteolyzed, monomeric subunit is 3.8 S. The present study was undertaken to determine which is the most common form generated both in vitro and in vivo and the structural relationship between these two forms. A wide variety of in vitro transformation protocols all yielded the 5.2S form when analyzed on molybdate-containing sucrose gradients by using a vertical tube rotor. Kinetic studies showed that the appearance of the 5.2S form coincided precisely with the appearance of transformed receptor, as defined by DEAE-cellulose elution. Furthermore, when the 3.8S and 5.2S peaks were collected from sucrose gradients directly, they were transformed receptors as defined by both DEAE-cellulose and DNA-cellulose chromatography, while the 9.1S sucrose gradient peak was untransformed when the same criteria were used. The 3.8S monomer, when isolated from high-salt sucrose gradients and then desalted, reverted to the 5.2S form (molybdate-containing gradients) or a 6.6S form (low-salt, molybdate-free gradients).(ABSTRACT TRUNCATED AT 250 WORDS)
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小牛肠道碱性磷酸酶、磷酸酶抑制剂和磷酸化化合物对糖皮质激素受体复合物激活率的影响。
影响因子：
2.9
作者：
Barnett,CA;Schmidt,TJ;Litwack,G
通讯作者：
Litwack,G
鉴定可将雄激素受体的 4.5S 形式转化为 8S 的 8S 雄激素受体促进因子。
影响因子：
4.8
作者：
Colvard,DS;Wilson,EM
通讯作者：
Wilson,EM
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Raaka,BM;Samuels,HH
通讯作者：
Samuels,HH
将调节剂重新定义为糖皮质激素受体激活的抑制剂。
DOI：
10.1016/0022-4731(81)90169-2
发表时间：
1981
期刊：
Journal of steroid biochemistry
影响因子：
--
作者：
Sekula,BC;Schmidt,TJ;Litwack,G
通讯作者：
Litwack,G