Effect of retinoic acid and interferon alpha-2a on transitional cell carcinoma of bladder.
Effect of retinoic acid and interferon alpha-2a on transitional cell carcinoma of bladder.
复制标题
视黄酸和干扰素α-2a对膀胱移行细胞癌的作用。
DOI：
10.1097/01.ju.0000141584.50650.26
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Liebert,Monica
中科院分区：
文献类型：
--
作者：
Zou,Changping;Ramakumar,Sanjay;Qian,Lixin;Zou,Changchun;Zang,Rongyu;Wang,Jian;Grossman,HBarton;Lotan,Reuben;Liebert,Monica
PurposeRetinoids modulate the growth and differentiation of normal and malignant epithelial cells in vitro and in vivo. Retinoids and their analogues have been used in animal models and clinical trials of chemoprevention and superficial bladder cancer treatment. Interferons are cytokines that have antiviral, antiproliferative and immunomodulatory function. They are used in many clinical trials for the treatment of different cancers. To identify new effective agents and develop novel approaches for the chemoprevention and treatment of superficial bladder cancer we investigated the effects of a combination of retinoids and interferon α-2a (IFN) on growth and apoptosis in bladder cancer cell lines.Materials and MethodsThe 4 bladder cancer cell lines UM-UC-6, UM-UC-9, UM-UC-10 and UM-UC-13 were treated with 2 retinoids, namely all-trans-retinoic acid (ATRA) and 9-cis retinoic acid (9cRA), as well as with IFN or with combinations of retinoids and IFN. The ability of these agents used alone and in combination to inhibit growth, induce apoptosis and modulate gene expression was investigated. The effects of retinoids on an INF related gene were also examined.ResultsMost bladder cancer cell lines were resistant to growth inhibition and apoptosis induction by ATRA and 9cRA, even at a high concentration. The effects of these retinoids on cell growth and apoptosis were enhanced by IFN. The combination of ATRA and IFN induced retinoic acid receptor β, and signal transducer and activator of transcription 1 expression in 3 bladder cancer cell lines, as detected by reverse transcriptase-polymerase chain reaction and Western blot analysis. Retinoids increased IFN-related gene expression detected by microarray analysis and real-time reverse transcriptase-polymerase chain reaction.ConclusionsThe results demonstrate that IFN acts synergistically with ATRA and 9cRA in the growth and apoptosis of bladder cancer cells in vitro and suggest that this combination has a potential for the treatment of transitional cell carcinoma of the bladder.
登录
查看更多内容
静息和激活的人中性粒细胞的内源性蛋白质磷酸化。
影响因子：
20.3
作者：
Andrews,PC;Babior,BM
通讯作者：
Babior,BM
常染色体慢性肉芽肿病中不存在两种胞质中性粒细胞氧化酶成分。
DOI：
10.1126/science.2848318
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Volpp,BD;Nauseef,WM;Clark,RA
通讯作者：
Clark,RA
Y.Kanaho 等人：“fMet-Leu-Phe 对兔中性粒细胞中磷脂酶 D 的激活是由百日咳毒素敏感的 GTP 结合蛋白介导的，该蛋白可能不同于磷脂酶 C 调节蛋白”FEBS Lett。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
蛋白质磷酸化和呼吸爆发。
影响因子：
3.9
作者：
Babior,BM
通讯作者：
Babior,BM
47 和 49kDa 蛋白质的磷酸化伴随着中性粒细胞释放超氧化物。
DOI：
10.1016/0006-291x(89)92825-8
发表时间：
1989
影响因子：
3.1
作者：
Badwey,JA;Heyworth,PG;Karnovsky,ML
通讯作者：
Karnovsky,ML