Octopamine neuromodulation regulates Gr32a-linked aggression and courtship pathways in Drosophila males.
Octopamine neuromodulation regulates Gr32a-linked aggression and courtship pathways in Drosophila males.
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DOI：
10.1371/journal.pgen.1004356
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发表时间：
2014-05
期刊：
影响因子：
4.5
通讯作者：
Certel SJ
中科院分区：
文献类型：
--
作者：
Andrews JC;Fernández MP;Yu Q;Leary GP;Leung AK;Kavanaugh MP;Kravitz EA;Certel SJ
化学感应信息素信息调节许多物种的攻击和繁殖，但信息素信号如何转导以可靠地产生行为尚不清楚。在这里，我们证明表达 Gr32a 的化学感应神经元检测到的增强雄性攻击性的信息素信号是通过章鱼胺（OA，去甲肾上腺素的无脊椎动物等效物）神经元过滤的。通过行为分析，我们发现缺乏章鱼胺和 Gr32a 味觉受体的雄性表现出攻击性发作的延迟和攻击性的减少。生理和解剖实验确定了 Gr32a 与食管下神经节中章鱼胺神经元突触和功能的连接。细化 Gr32a 表达群体表明，口腔 Gr32a 神经元促进雄性攻击性并与 OA 神经元形成突触接触。通过限制单胺神经元目标群体，我们发现三个先前识别的参与行为选择的 OA-FruM 神经元位于 Gr32a-OA 连接之中。我们的研究结果表明，章鱼胺能神经调节神经元早在化学感应驱动的男性社会行为途径的第二阶步骤中就发挥作用。交配还是打斗？当遇到其物种的其他成员时，雄性果蝇必须确定第二只果蝇是雄性还是雌性，并采取适当的行为模式。据报道，味觉受体 Gr32a 会对对性别识别很重要的化学信息（信息素）做出反应，因为消除 Gr32a 功能会损害雄性求爱和攻击行为。在这里，我们证明表达 Gr32a 的神经元群体的不同子集介导这些相互排斥的行为，并且雄性 Gr32a 介导的行为反应通过含有神经调节剂章鱼胺（OA，去甲肾上腺素的无脊椎动物等效物）的神经元被放大。表达 Gr32a 的神经元与不同的 OA 神经元在功能上和突触上连接，表明这些胺神经元可能早在化学感应驱动电路的二阶步骤中就发挥作用。我们的结果有助于理解生物体在接收外部感官信号时如何选择适当的行为反应。
Chemosensory pheromonal information regulates aggression and reproduction in many species, but how pheromonal signals are transduced to reliably produce behavior is not well understood. Here we demonstrate that the pheromonal signals detected by Gr32a-expressing chemosensory neurons to enhance male aggression are filtered through octopamine (OA, invertebrate equivalent of norepinephrine) neurons. Using behavioral assays, we find males lacking both octopamine and Gr32a gustatory receptors exhibit parallel delays in the onset of aggression and reductions in aggression. Physiological and anatomical experiments identify Gr32a to octopamine neuron synaptic and functional connections in the suboesophageal ganglion. Refining the Gr32a-expressing population indicates that mouth Gr32a neurons promote male aggression and form synaptic contacts with OA neurons. By restricting the monoamine neuron target population, we show that three previously identified OA-FruM neurons involved in behavioral choice are among the Gr32a-OA connections. Our findings demonstrate that octopaminergic neuromodulatory neurons function as early as a second-order step in this chemosensory-driven male social behavior pathway. To mate or fight? When meeting other members of their species, male fruit flies must determine whether a second fly is male or female and proceed with the appropriate behavioral patterns. The taste receptor, Gr32a, has been reported to respond to chemical messages (pheromones) that are important for gender recognition, as eliminating Gr32a function impairs both male courtship and aggressive behavior. Here we demonstrate that different subsets of Gr32a-expressing neuron populations mediate these mutually exclusive behaviors and the male Gr32a-mediated behavioral response is amplified through neurons that contain the neuromodulator octopamine (OA, an invertebrate equivalent of norepinephrine). Gr32a-expressing neurons connect functionally and synaptically with distinct OA neurons indicating these amine neurons may function as early as a second-order step in a chemosensory-driven circuit. Our results contribute to understanding how an organism selects an appropriate behavioral response upon receiving external sensory signals.
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影响因子：
2.5
作者：
Busch, Sebastian;Selcho, Mareike;Tanimoto, Hiromu
通讯作者：
Tanimoto, Hiromu
影响因子：
16.2
作者：
Delaney, Andrew J.;Crane, James W.;Sah, Pankaj
通讯作者：
Sah, Pankaj
影响因子：
9.2
作者：
Chiang, Ann-Shyn;Lin, Chih-Yung;Hwang, Jenn-Kang
通讯作者：
Hwang, Jenn-Kang
影响因子：
16.2
作者：
Gordon, Michael D.;Scott, Kristin
通讯作者：
Scott, Kristin
DOI：
10.1007/s00359-013-0851-5
发表时间：
2013-11
期刊：
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
影响因子：
--
作者：
Fernández MP;Kravitz EA
通讯作者：
Kravitz EA