Radio-frequency-free cell for electron capture dissociation in tandem mass spectrometry.
Radio-frequency-free cell for electron capture dissociation in tandem mass spectrometry.
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DOI：
10.1021/ac802084w
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发表时间：
2009-02-01
影响因子：
7.4
通讯作者：
Barofsky DF
中科院分区：
文献类型：
--
作者：
Voinov VG;Deinzer ML;Barofsky DF
一个射频自由（RFF），分析仪独立的细胞已被设计为电子捕获解离（ECD）的离子。该装置是基于交错的一系列静电透镜与静磁透镜的周期性结构中常见的行波管。RFF静电/静磁ECD单元安装在Finnigan TSQ700 ESI三重四极杆（QqQ）光谱仪，其性能进行了评价，通过记录产物离子光谱的双质子化物质P，双质子化短杆菌肽S，双质子化神经降压素，和三重质子化神经降压素。这些光谱很容易获得，而无需求助于缓冲气体或同步电子注入与RF场的特定相位。用改进的仪器产生的质谱在所有方面（除了分辨率和质量准确度，这是由所使用的质谱仪的限制），至少是一样好的肽鉴定的目的，那些记录与傅里叶变换离子回旋共振（FT ICR）仪器;然而，产生质谱的努力和时间远远少于所需的产生其FT ICR对应物。该池的设计和紧凑的构造应允许其以相对低的成本并入到几乎任何类型的串联质谱仪中，例如，三重四极杆、混合四极杆离子阱、混合四极杆飞行时间或甚至FT-ICR。
A radio frequency-free (RFF), analyzer-independent cell has been devised for electron-capture dissociation (ECD) of ions. The device is based on interleaving a series of electrostatic lenses with the periodic structure of magnetostatic lenses commonly found in a traveling wave tube. The RFF electrostatic/magnetostatic ECD cell was installed in a Finnigan TSQ700 ESI triple quadrupole (QqQ) spectrometer, and its performance was evaluated by recording product-ion spectra of doubly protonated substance P, doubly protonated gramicidin S, doubly protonated neurotensin, and triply protonated neurotensin. These spectra were readily obtained without recourse to a buffering gas or synchronizing electron injection with a specific phase of an RF field. The mass spectra produced with the modified instrument appear in all respects (other than resolution and mass accuracy, which were limited by the mass spectrometer used) to be at least as good for purposes of peptide identification as those recorded with Fourier transform ion cyclotron resonance (FT ICR) instruments; however, the effort and time to produce the mass spectra were much less than required to produce their FT ICR counterparts. The cell’s design and compact construction should allow it to be incorporated at relatively little cost into virtually any type of tandem mass spectrometer, for example, triple quadrupole, hybrid quadrupole ion trap, hybrid quadrupole time-of-flight, or even FT-ICR.
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DOI：
10.1016/j.jasms.2008.05.015
发表时间：
2008-09
影响因子：
3.2
作者：
Creese AJ;Cooper HJ
通讯作者：
Cooper HJ
影响因子：
7.4
作者：
Mirgorodskaya, E;Roepstorff, P;Zubarev, RA
通讯作者：
Zubarev, RA
影响因子：
7.4
作者：
Håkansson, K;Emmett, MR;Marshall, AG
通讯作者：
Marshall, AG
影响因子：
4.4
作者：
Chalmers, MJ;Quinn, JP;Marshall, AG
通讯作者：
Marshall, AG
DOI：
10.1016/s1044-0305(00)00223-3
发表时间：
2001-03-01
影响因子：
3.2
作者：
McLafferty, FW;Horn, DM;Carpenter, BK
通讯作者：
Carpenter, BK