Differences between naive and memory T cell phenotype in Malawian and UK adolescents: a role for Cytomegalovirus?
Differences between naive and memory T cell phenotype in Malawian and UK adolescents: a role for Cytomegalovirus?
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DOI：
10.1186/1471-2334-8-139
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发表时间：
2008-10-15
影响因子：
3.7
通讯作者：
Dockrell HM
中科院分区：
文献类型：
--
作者：
Ben-Smith A;Gorak-Stolinska P;Floyd S;Weir RE;Lalor MK;Mvula H;Crampin AC;Wallace D;Beverley PC;Fine PE;Dockrell HM
已假设早期生活中环境暴露于抗原的程度的差异导致来自不同人群的个体的免疫状态的差异，这可能对以后的免疫应答有影响。收集HIV阴性青少年的静脉血和HIV阴性妇女分娩后婴儿脐带血，并使用流式细胞术进行分析。从外周血淋巴细胞和巨细胞病毒（CMV）血清阳性的青少年T细胞表型进行了测定，通过ELISA进行评估。与年龄匹配的英国青少年相比，HIV阴性的马拉维青少年具有较低的幼稚T细胞（CD 45 RO-CD 62 Lhi CD 11 alo）百分比，较高的记忆T细胞比例和较高的CD 28-记忆（CD 28-CD 45 RO+）T细胞百分比。与英国青少年相比，马拉维青少年的中央记忆（CD 45 RA + CCR 7+）T细胞百分比较低，而稳定记忆（CD 45 RA + CCR 7-）T细胞百分比较高。在马拉维接受测试的所有青少年均为CMV血清阳性（59/59），而英国青少年为21/58（36%）。英国的CMV血清阳性与外周血幼稚T细胞百分比降低和CD 28记忆T细胞百分比增加相关。在两个研究中心的脐带血样本中未观察到幼稚和记忆T细胞群的比例存在差异。马拉维和英国青少年之间的这些差异很可能反映了非洲环境中对各种感染（包括CMV）的自然暴露程度更高，并可能意味着这些人群诱导和维持对疫苗和自然感染的免疫记忆的能力存在差异。
Differences in degree of environmental exposure to antigens in early life have been hypothesized to lead to differences in immune status in individuals from different populations, which may have implications for immune responses in later years. Venous blood from HIV-negative adolescents and blood from the umbilical cords of babies, born to HIV-negative women, post-delivery was collected and analysed using flow cytometry. T cell phenotype was determined from peripheral blood lymphocytes and cytomegalovirus (CMV) seropositivity was assessed by ELISA in adolescents. HIV-negative Malawian adolescents were shown to have a lower percentage of naïve T cells (CD45RO-CD62Lhi CD11alo), a higher proportion of memory T cells and a higher percentage of CD28- memory (CD28-CD45RO+) T cells compared to age-matched UK adolescents. Malawian adolescents also had a lower percentage of central memory (CD45RA-CCR7+) T cells and a higher percentage of stable memory (CD45RA+CCR7-) T cells than UK adolescents. All of the adolescents tested in Malawi were seropositive for CMV (59/59), compared to 21/58 (36%) of UK adolescents. CMV seropositivity in the UK was associated with a reduced percentage of naïve T cells and an increased percentage of CD28- memory T cells in the periphery. No differences in the proportions of naïve and memory T cell populations were observed in cord blood samples from the two sites. It is likely that these differences between Malawian and UK adolescents reflect a greater natural exposure to various infections, including CMV, in the African environment and may imply differences in the ability of these populations to induce and maintain immunological memory to vaccines and natural infections.
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影响因子：
168.9
作者：
Black, GF;Weir, RE;Dockrell, HM
通讯作者：
Dockrell, HM
影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
影响因子：
168.9
作者：
Fine, PEM;Ponnighaus, JM;Peto, R
通讯作者：
Peto, R
影响因子：
64.8
作者：
Champagne, P;Ogg, GS;Pantaleo, G
通讯作者：
Pantaleo, G
影响因子：
4.6
作者：
Kassu, A;Tsegaye, A;De Wit, TFR
通讯作者：
De Wit, TFR