Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses.
Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses.
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DOI：
10.1371/journal.pone.0064193
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Saban DR
中科院分区：
文献类型：
--
作者：
Khandelwal P;Blanco-Mezquita T;Emami P;Lee HS;Reyes NJ;Mathew R;Huang R;Saban DR
Steady state dendritic cells (DC) found in non-lymphoid tissue sites under normal physiologic conditions play a pivotal role in triggering T cell responses upon immune provocation. CD11b+ and CD103+ DC have received considerable attention in this regard. However, still unknown is whether such CD11b+ and CD103+ DC even exist in the ocular mucosa, and if so, what functions they have in shaping immune responses. We herein identified in the ocular mucosa of normal wild-type (WT) and Flt3-/- mice the presence of a CD11b+ DC (i.e., CD11c+ MHCII+ CD11b+ CD103- F4/80+ Sirp-a+). CD103+ DC (i.e. CD11c+ MHCII+ CD11b low CD103+ CD8a+ DEC205+ Langerin+) were also present in WT, but not in Flt3-/- mice. These CD103+ DC expressed high levels of Id2 and Flt3 mRNA; whereas CD11b+ DC expressed high Irf4, Csfr, and Cx3cr1 mRNA. Additionally, the functions of these DC differed in response to allergic immune provocation. This was assessed utilizing a previously validated model, which includes transferring specific populations of exogenous DC into the ocular mucosa of ovalbumin (OVA)/alum-primed mice. Interestingly, in such mice, topical OVA instillation following engraftment of exogenous CD11b+ DC led to dominant allergic T cell responses and clinical signs of ocular allergy relative to those engrafted with CD103+ DC. Thus, although CD11b+ and CD103+ DC are both present in the normal ocular mucosa, the CD11b+ DC subset plays a dominant role in a mouse model of ocular allergy.
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影响因子：
30.5
作者：
Bedoui, Sammy;Whitney, Paul G.;Heath, William R.
通讯作者：
Heath, William R.
影响因子：
3.6
作者：
Ohbayashl, Masaharu;Manzouri, Bita;Ono, Santa Jeremy
通讯作者：
Ono, Santa Jeremy
影响因子：
2.2
作者：
Lutz, MB;Kukutsch, N;Schuler, G
通讯作者：
Schuler, G
DOI：
10.1084/jem.20091925
发表时间：
2009-12-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Schulz O;Jaensson E;Persson EK;Liu X;Worbs T;Agace WW;Pabst O
通讯作者：
Pabst O
影响因子：
6
作者：
Schlereth, Simona;Lee, Hyun Soo;Saban, Daniel R.
通讯作者：
Saban, Daniel R.