Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis.
Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis.
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DOI：
10.1099/ijsem.0.004403
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发表时间：
2020-10
影响因子：
2.8
通讯作者：
Beemelmanns C
中科院分区：
文献类型：
--
作者：
Benndorf R;Martin K;Küfner M;de Beer ZW;Vollmers J;Kaster AK;Beemelmanns C
用多相方法确定了两株新的好氧、革兰氏阳性放线杆菌，命名为菌株RB29T和RB68T的分类地位。根据16SRRNA基因序列分析，RB29T的系统发育近邻分别为放线放线放线菌DSM 102126T(同源性99.2 %)和放线放线菌DSM 43919T(98.7 %)，菌株RB68T为放线放线菌DSM 44148T(98.3 %)。Rb29T与其亲缘关系最近的类群A.rayongensis DSM102126T和A.atatmentaria DSM43919T的数字杂交相似性分别为53.2 %(50.6-55.9 %)和26.4 %(24.1-28.9 %)。平均核苷酸同源性分别为93.2 %(94.0 %)和82.3 %(78.9 %)。菌株RB68T与木霉DSM 44148T的dDDH相似性为24.5 %(22.2-27.0 %)。两株放线菌RB29T和RB68T都表现出典型的放线菌属的形态特征和化学分类特征，如细胞壁中存在中位二氨基二苯酸，全细胞水解物中的主要糖组分为半乳糖和葡萄糖，不含真菌酸。主要磷脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。RB29T以MK-9(H6)和MK-9(H8)为主，RB68T以MK-9(H4)和MK-9(H6)为主。其中主要脂肪酸为10-甲基十五烷酸(10-甲基C18：0)、14-甲基十五烷酸(异-C16：0)、十六烷酸(C16：0)和顺-9-十八烷酸(C18 ： 1ω9c)。在此，我们提出了两个新种：放线虫Actinomadura ruteroloni sp.11月模式菌株RB29T(=CCUG 72668T=NRRL B-65537T)和大白蚁放线菌。11月模式菌株RB68T(=CCUG 72669T=NRRL B-65538T)。
The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, designated strains RB29T and RB68T, were determined using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of RB29T were identified as Actinomadura rayongensis DSM 102126T (99.2 % similarity) and Actinomadura atramentaria DSM 43919T (98.7 %), and for strain RB68T was Actinomadura hibisca DSM 44148T (98.3 %). Digital DNA–DNA hybridization (dDDH) between RB29T and its closest phylogenetic neighbours, A. rayongensis DSM 102126T and A. atramentaria DSM 43919T, resulted in similarity values of 53.2 % (50.6–55.9 %) and 26.4 % (24.1–28.9 %), respectively. Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for A. rayongensis DSM 102126T and 82.3 % (78.9 %) for A. atramentaria DSM 43919T. dDDH analysis between strain RB68T and A. hibisca DSM 44148T gave a similarity value of 24.5 % (22.2–27.0 %). Both strains, RB29T and RB68T, revealed morphological characteristics and chemotaxonomic features typical for the genus Actinomadura , such as the presence of meso-diaminopimelic acid in the cell wall, galactose and glucose as major sugar components within whole-cell hydrolysates and the absence of mycolic acids. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Predominant menaquinones were MK-9(H6) and MK-9(H8) for RB29T and MK-9(H4) and MK-9(H6) for RB68T. The main fatty acids were identified as 10-methyloctadecanoic acid (10-methyl C18:0), 14-methylpentadecanoic acid (iso-C16:0), hexadecanoic acid (C16:0) and cis-9-octadecanoic acid (C18 : 1 ω9c). Here, we propose two novel species of the genus Actinomadura : Actinomadura rubteroloni sp. nov. with the type strain RB29T (=CCUG 72668T=NRRL B-65537T) and Actinomadura macrotermitis sp. nov. with the type strain RB68T (=CCUG 72669T=NRRL B-65538T).
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DOI：
10.1099/00221287-100-2-221
发表时间：
1977-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
COLLINS, MD;PIROUZ, T;MINNIKIN, DE
通讯作者：
MINNIKIN, DE
DOI：
10.1099/ijs.0.056994-0
发表时间：
2014-02-01
影响因子：
2.8
作者：
Meier-Kolthoff, Jan P.;Klenk, Hans-Peter;Goeker, Markus
通讯作者：
Goeker, Markus
影响因子：
2.8
作者：
Meier-Kolthoff, Jan P.;Goeker, Markus;Klenk, Hans-Peter
通讯作者：
Klenk, Hans-Peter
影响因子：
3
作者：
Meier-Kolthoff JP;Auch AF;Klenk HP;Göker M
通讯作者：
Göker M
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro