Green electrospinning for biomaterials and biofabrication.
Green electrospinning for biomaterials and biofabrication.
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用于生物材料和生物制造的绿色静电纺丝。
DOI：
10.1088/1758-5090/ac0964
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发表时间：
2021-06-28
期刊：
影响因子：
9
通讯作者：
Lu HH
中科院分区：
文献类型：
--
作者：
Mosher CZ;Brudnicki PAP;Gong Z;Childs HR;Lee SW;Antrobus RM;Fang EC;Schiros TN;Lu HH
由于人们日益认识到传统做法对环境和健康的负面影响，各行业都出现了绿色制造。在生物材料行业中，静电纺丝是一种普遍存在的制造方法，用于生产类似于天然组织的纳米至微米级纤维网，但该工艺传统上使用对环境有害的溶剂，并对工业规模扩大和临床转化构成重大障碍。将可持续性原则应用于生物材料生产，我们通过系统地测试生物良性溶剂（美国食品和药物管理局Q3 C 3类）开发了“绿色静电纺丝”工艺，并将乙酸鉴定为表现出低生态影响（全球变暖潜势（GWP）= 1.40 CO2 eq.）的绿色溶剂。kg/L），并在常规制造条件下支持稳定的静电纺丝射流。通过调整静电纺丝参数，如针板距离和流速，我们更新了广泛使用的生物医学聚合物（如聚-α-羟基酯，胶原蛋白），聚合物共混物，聚合物陶瓷复合材料和生长因子输送系统的制造。所得“绿色”纤维和复合材料在成分、化学、结构、机械性能和生物相容性方面与传统补片相当。有趣的是，绿色合成纤维的材料性能比传统的静电纺丝纤维更仿生，延展性加倍（91.86 ± 35.65 vs. 45 ± 15.07%，n = 10，p < 0.05），而不影响屈服强度（1.32 ± 0.26对比1.38 ± 0.32 MPa）或极限拉伸强度（2.49 ± 0.55对比2.36 ± 0.45 MPa）。最重要的是，绿色静电纺丝被证明对生物织物有利，在纤维形成期间对生长因子提供更大的保护（72.30 ± 1.94 vs. 62.87 ± 2.49% α螺旋含量，n = 3，p < 0.05），并概括了生物聚合物基网片制造中的天然ECM力学（16.57 ± 3.92%延展性，33.38 ± 30.26 MPa弹性模量，1.30 ± 0.19 MPa屈服强度和2.13 ± 0.36 MPa极限拉伸强度，n = 10）。这里展示的生态意识方法代表了生物制造业的范式转变，并将加速可扩展生物材料和仿生支架在组织工程和再生医学中的转化。
Green manufacturing has emerged across industries, propelled by a growing awareness of the negative environmental and health impacts associated with traditional practices. In the biomaterials industry, electrospinning is a ubiquitous fabrication method for producing nano- to micro-scale fibrous meshes that resemble native tissues, but this process traditionally utilizes solvents that are environmentally hazardous and pose a significant barrier to industrial scale-up and clinical translation. Applying sustainability principles to biomaterial production, we have developed a ‘green electrospinning’ process by systematically testing biologically benign solvents (U.S. Food and Drug Administration Q3C Class 3), and have identified acetic acid as a green solvent that exhibits low ecological impact (global warming potential (GWP) = 1.40 CO2 eq. kg/L) and supports a stable electrospinning jet under routine fabrication conditions. By tuning electrospinning parameters, such as needle-plate distance and flow rate, we updated the fabrication of widely utilized biomedical polymers (e.g. poly-α-hydroxyesters, collagen), polymer blends, polymer-ceramic composites, and growth factor delivery systems. Resulting ‘green’ fibers and composites are comparable to traditional meshes in terms of composition, chemistry, architecture, mechanical properties, and biocompatibility. Interestingly, material properties of green synthetic fibers are more biomimetic than those of traditionally electrospun fibers, doubling in ductility (91.86 ± 35.65 vs. 45 ± 15.07%, n = 10, p < 0.05) without compromising yield strength (1.32 ± 0.26 vs. 1.38 ± 0.32 MPa) or ultimate tensile strength (2.49 ± 0.55 vs. 2.36 ± 0.45 MPa). Most importantly, green electrospinning proves advantageous for biofabrication, rendering a greater protection of growth factors during fiber formation (72.30 ± 1.94 vs. 62.87 ± 2.49% alpha helical content, n = 3, p < 0.05) and recapitulating native ECM mechanics in the fabrication of biopolymer-based meshes (16.57 ± 3.92% ductility, 33.38 ± 30.26 MPa elastic modulus, 1.30 ± 0.19 MPa yield strength, and 2.13 ± 0.36 MPa ultimate tensile strength, n = 10). The eco-conscious approach demonstrated here represents a paradigm shift in biofabrication, and will accelerate the translation of scalable biomaterials and biomimetic scaffolds for tissue engineering and regenerative medicine.
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