Inhibition of the Plastidic ATP/ADP Transporter Protein Primes Potato Tubers for Augmented Elicitation of Defense Responses and Enhances Their Resistance against Erwinia carotovora
Inhibition of the Plastidic ATP/ADP Transporter Protein Primes Potato Tubers for Augmented Elicitation of Defense Responses and Enhances Their Resistance against Erwinia carotovora
复制标题
质体 ATP/ADP 转运蛋白的抑制可增强马铃薯块茎的防御反应并增强其对胡萝卜软腐欧文氏菌的抵抗力
DOI：
10.1104/pp.000802
复制
发表时间：
2002
期刊：
影响因子：
7.4
通讯作者：
H. Neuhaus
中科院分区：
文献类型：
--
作者：
C. Linke;U. Conrath;W. Jeblick;T. Betsche;A. Mahn;K. Düring;H. Neuhaus
质体ATP/ADP转运蛋白AATP 1活性降低的转基因马铃薯（Solanum tuberosum）植株的块茎显示淀粉水平降低、块茎形态改变和初级代谢产物浓度改变。在这里，我们证明了自发产生的过氧化氢，水杨酸的内源性含量，和各种防御相关基因的mRNA水平是相似的野生型和AATP 1（St）反义植物的块茎光盘。然而，在挑战的组织与真菌诱导子或培养上清液的软腐病病原体欧文氏菌carotovora亚种。atroseptica，AATP 1（St）反义块茎表现出高度增强激活的防御反应相比，野生型组织。增强的防御反应包括五种防御相关基因（β-1，3-葡聚糖酶B2和A1、几丁质酶B3和A2以及Phe氨裂解酶）转录物的增强积累和早期过氧化氢爆发的增强激发（高达21倍）。AATP 1（St）反义块茎中细胞防御反应的增强激活并不伴随着内源水杨酸水平的预先增加，而是与组织对E.胡萝卜素。从这些结果中，我们得出结论，初级代谢反应的抑制诱导引发的状态，敏感的马铃薯块茎，提高各种细胞防御反应，这可能有助于增强E。胡萝卜素抗性
Tubers of transgenic potato (Solanum tuberosum) plants with decreased activity of the plastidic ATP/ADP transporter AATP1 display reduced levels of starch, modified tuber morphology, and altered concentrations of primary metabolites. Here, we demonstrate that the spontaneous production of hydrogen peroxide, the endogenous content of salicylic acid, and the levels of mRNAs of various defense-related genes are similar in tuber discs of wild-type and AATP1(St) antisense plants. However, upon challenging the tissue with fungal elicitors or culture supernatants of the soft rot-causing pathogenErwinia carotovora subsp. atroseptica, the AATP1(St) antisense tubers exhibit highly potentiated activation of defense responses when compared with wild-type tissue. The augmented defense responses comprise enhanced accumulation of transcripts of five defense-related genes (β-1,3-GLUCANASE B2 andA1, CHITINASE B3 and A2, and Phe AMMONIA-LYASE) and enhanced elicitation (up to 21-fold) of the early hydrogen peroxide burst. The potentiated activation of cellular defense responses inAATP1(St) antisense tubers is not accompanied by a precedent increase in endogenous salicylic acid levels, but is associated with a strongly enhanced resistance of the tissue to E. carotovora. From these results, we conclude that inhibition of primary metabolic reactions induces a primed state that sensitizes the potato tubers for improved elicitation of various cellular defense responses, which likely contribute to enhancedE. carotovora resistance.
DOI：
10.1073/pnas.95.13.7819
发表时间：
1998-06-23
影响因子：
11.1
作者：
Yu, IC;Parker, J;Bent, AF
通讯作者：
Bent, AF