Strategies for engineering human chromosomes with therapeutic potential
Strategies for engineering human chromosomes with therapeutic potential
复制标题
具有治疗潜力的人类染色体工程策略
DOI：
--
复制
发表时间：
2002
影响因子：
3.5
通讯作者：
K. H. Andy Choo
中科院分区：
文献类型：
--
作者：
R. Saffery;K. H. Andy Choo
人类工程化染色体（HEC）相对于目前用于基因治疗应用的载体具有几个潜在的优点。首先，可以在这些载体中克隆的DNA没有大小上限。其次，它们的染色体外性质确保了引入的基因既不破坏宿主细胞的基因组，也不受其影响。最后，由于HEC载体仅来源于人，因此不应引起不利的宿主免疫原性应答。最近的进展已经通过几种不同的方法产生了各种HEC。本文综述了目前HEC载体的制备方法，每种方法的优点和存在的问题，并讨论了HEC载体作为离体治疗药物的前景。版权所有© 2001约翰威利父子有限公司。
Human engineered chromosomes (HECs) have several potential advantages over currently used vectors for gene therapy applications. Firstly, there is no upper size limit to DNA that can be cloned in these vectors. Secondly, their extrachromosomal nature ensures that introduced genes are neither disruptive to, nor affected by, the genome of the host cell. Finally, being solely human in origin, HEC vectors should not evoke adverse host immunogenic responses. Recent advances have produced a variety of HECs via several different approaches. This review focuses on the current methodologies for making HEC vectors, the advantages and problems associated with each strategy, and discusses the outlook for HEC vectors as ex vivo therapeutic agents. Copyright © 2001 John Wiley & Sons, Ltd.
影响因子：
4.2
作者：
H. Temin
通讯作者：
H. Temin
DOI：
10.1073/pnas.85.18.6622
发表时间：
1988-09-01
影响因子：
11.1
作者：
MOYZIS, RK;BUCKINGHAM, JM;WU, JR
通讯作者：
WU, JR