Intravitreal Stanniocalcin-1 Enhances New Blood Vessel Growth in a Rat Model of Laser-Induced Choroidal Neovascularization.
Intravitreal Stanniocalcin-1 Enhances New Blood Vessel Growth in a Rat Model of Laser-Induced Choroidal Neovascularization.
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DOI：
10.1167/iovs.17-23083
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发表时间：
2018-02-01
影响因子：
4.4
通讯作者：
Rosa RH Jr
中科院分区：
文献类型：
--
作者：
Zhao M;Xie W;Tsai SH;Hein TW;Rocke BA;Kuo L;Rosa RH Jr
本研究的目的是研究斯钙素-1（STC-1），一种光受体保护性糖蛋白，对激光损伤后脉络膜新生血管（CNV）的发展与VEGF及其主要受体（VEGFR 2）表达的关系。在大鼠中，通过激光光凝法在双眼中诱导CNV，然后在右眼中玻璃体内注射STC-1，并在左眼中注射载体或变性STC-1作为对照。激光损伤后2周行眼底自发荧光（FAF）成像和荧光素眼底血管造影（FFA）检查。使用定义的量表系统对CNV的荧光素渗漏进行分级。用光谱域光学相干断层扫描（SD-OCT）、荧光素标记的脉络膜-巩膜平片和苏木精-伊红染色定量CNV的大小。Western blot检测蛋白表达。光凝产生了一个界限分明的CNV区域。用STC-1治疗，通过FAF评估的CNV病变的强度和面积均增加50%。SD-OCT、平贴和组织学分析的CNV病变也增加。FFA显示STC-1治疗眼CNV病变中荧光素渗漏增强。STC-1蛋白在脉络膜组织中检测到，其水平随着CNV病变的增加而增加，并且与VEGF和VEGFR 2的表达相关。玻璃体内施用STC-1显著增加VEGF和VEGFR 2蛋白的脉络膜表达。脉络膜组织表达STC-1，其似乎通过增强激光诱导的CNV中的病理性新血管生长而作为应激反应蛋白。STC-1可能通过VEGF信号传导促进CNV的发展。
The purpose of this study was to investigate the impact of stanniocalcin-1 (STC-1), a photoreceptor-protective glycoprotein, on the development of choroidal neovascularization (CNV) in relation to VEGF and its main receptor (VEGFR2) expression after laser injury. In rats, CNV was induced by laser photocoagulation in both eyes, followed by intravitreal injection of STC-1 in the right eye and vehicle or denatured STC-1 injection in the left eye as control. Two weeks after laser injury, fundus autofluorescence (FAF) imaging and fundus fluorescein angiography (FFA) were performed. Fluorescein leakage from CNV was graded using a defined scale system. The size of CNV was quantified with spectral domain optical coherence tomography (SD-OCT), fluorescein-labeled choroid-sclera flat mounts, and hematoxylin-eosin staining. Protein expressions were evaluated by Western blot. Photocoagulation produced a well-circumscribed area of CNV. With STC-1 treatment, CNV lesions assessed by FAF were increased by 50% in both intensity and area. The CNV lesions were also increased with SD-OCT, flat-mount, and histologic analyses. FFA disclosed enhanced fluorescein leakage in CNV lesions in STC-1 treated eyes. The STC-1 protein was detected in the choroidal tissue and its level was increased with CNV lesions in correlation with VEGF and VEGFR2 expressions. Intravitreal administration of STC-1 significantly increased choroidal expression of both VEGF and VEGFR2 proteins. Chorodial tissue expresses STC-1, which seemingly acts as a stress response protein by enhancing pathological new blood vessel growth in laser-induced CNV. It is likely that STC-1 promotes CNV development via VEGF signaling.
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DOI：
10.3791/51018
发表时间：
2013-12-13
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Ivanova E;Toychiev AH;Yee CW;Sagdullaev BT
通讯作者：
Sagdullaev BT
Stanniocalcin-1通过上调胃癌细胞中的VEGF促进肿瘤血管生成
影响因子：
11
作者：
He LF;Wang TT;Gao QY;Zhao GF;Huang YH;Yu LK;Hou YY
通讯作者：
Hou YY
影响因子：
4.6
作者：
Gerritsen, ME;Soriano, R;Williams, PM
通讯作者：
Williams, PM
影响因子：
2.7
作者：
LAFEBER, FPJG;HANSSEN, RGJM;BONGA, SEW
通讯作者：
BONGA, SEW
DOI：
10.1073/pnas.93.5.1792
发表时间：
1996-03-05
影响因子：
11.1
作者：
Olsen, HS;Cepeda, MA;Wagner, GF
通讯作者：
Wagner, GF