Antigen‐binding human T suppressor cells and their association with the HLA‐DR locus
Antigen‐binding human T suppressor cells and their association with the HLA‐DR locus
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抗原结合人 T 抑制细胞及其与 HLA-DR 基因座的关联
DOI：
10.1002/eji.1830130505
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发表时间：
1983
影响因子：
5.4
通讯作者：
T. Lehner
中科院分区：
文献类型：
--
作者：
T. Lehner
通过直接结合125 I标记的链球菌蛋白抗原，然后进行放射自显影，研究了人淋巴细胞结合抗原的能力。去除粘附细胞和B细胞的T富集淋巴细胞在4°C和存在叠氮化钠的情况下显示出125 I标记的链球菌抗原（SA）的特异性结合。通过单克隆T4或T8抗血清和补体进一步消耗富含T细胞的群体，显示抗原结合T细胞是T4−，T8+。通过T8细胞的阳性选择、与牛红细胞的玫瑰花结以及通过体外诱导的抑制细胞而非辅助细胞与SA的结合证实了这一点。通过SA完全抑制而匙孔血蓝蛋白不抑制来建立与抑制细胞结合的抗原特异性。发现1或10 ng SA与HLA-DRw 6淋巴细胞结合以及1000 ng SA与DR 4、1、2、3或5淋巴细胞结合的特征性剂量反应。将抗原结合T+抑制细胞与辅助和抑制功能的剂量反应进行比较表明，与抑制细胞结合的SA剂量与诱导辅助功能而非抑制功能所需的剂量相似。对这些观察结果的一个合理解释是，T8+抗原结合抑制细胞可能起着“反抑制细胞”的作用，它成功地竞争辅助细胞的膜受体，从而防止抑制细胞的主要亚群的抑制。
The ability of human lymphocytes to bind antigen was studied by direct binding of 125I‐labeled streptococcal protein antigen, followed by autoradiography. T‐enriched lymphocytes depleted of adherent cells and B cells showed specific binding of 125I‐labeled streptococcal antigen (SA) at 4°C and in the presence of sodium azide. Further depletions of the T‐enriched population by the monoclonal T4 or T8 antiserum and complement revealed that the antigen‐binding T cell is T4−, T8+. This was confirmed by positive selection of T8 cells, by rosetting with ox red blood cells and by the binding of SA by in vitro induced suppressor but not helper cells. Antigen specificity of binding to the suppressor cells was established by complete inhibition with the SA but no inhibition with keyhole limpet hemocyanin. A characteristic dose‐response of binding 1 or 10 ng SA to HLA‐DRw6 lymphocytes and 1000 ng SA to DR4,1,2,3 or 5 lymphocytes was found. A comparison of the dose‐responses of antigen‐binding T+ suppressor cells with those of helper and suppressor functions showed that the dose of SA which binds to suppressor cells is similar to the dose required to induce helper but not suppressor function. A plausible interpretation of these observations is that the T8+ antigen‐binding suppressor cells might function as “contrasuppressor cells” which compete successfully for the membrane receptors of helper cells, thereby preventing suppression by the major subset of suppressor cells.
隐球菌特异性抑制性 T 细胞因子的免疫吸附。
影响因子：
3.1
作者：
Mosley,RL;Murphy,JW;Cox,RA
通讯作者：
Cox,RA