Novel, divergent simian hemorrhagic fever viruses in a wild Ugandan red colobus monkey discovered using direct pyrosequencing.
Novel, divergent simian hemorrhagic fever viruses in a wild Ugandan red colobus monkey discovered using direct pyrosequencing.
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使用直接焦磷酸测序发现的野生乌干达红色糖果猴中的新颖的，不同的猿猴出血性发烧病毒。
DOI：
10.1371/journal.pone.0019056
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发表时间：
2011-04-22
期刊：
影响因子：
3.7
通讯作者：
Goldberg TL
中科院分区：
文献类型：
--
作者：
Lauck M;Hyeroba D;Tumukunde A;Weny G;Lank SM;Chapman CA;O'Connor DH;Friedrich TC;Goldberg TL
Simian hemorrhagic fever virus (SHFV) has caused lethal outbreaks of hemorrhagic disease in captive primates, but its distribution in wild primates has remained obscure. Here, we describe the discovery and genetic characterization by direct pyrosequencing of two novel, divergent SHFV variants co-infecting a single male red colobus monkey from Kibale National Park, Uganda. The viruses were detected directly from blood plasma using pyrosequencing, without prior virus isolation and with minimal PCR amplification. The two new SHFV variants, SHFV-krc1 and SHFV-krc2 are highly divergent from each other (51.9% nucleotide sequence identity) and from the SHFV type strain LVR 42-0/M6941 (52.0% and 51.8% nucleotide sequence identity, respectively) and demonstrate greater phylogenetic diversity within SHFV than has been documented within any other arterivirus. Both new variants nevertheless have the same 3′ genomic architecture as the type strain, containing three open reading frames not present in the other arteriviruses. These results represent the first documentation of SHFV in a wild primate and confirm the unusual 3′ genetic architecture of SHFV relative to the other arteriviruses. They also demonstrate a degree of evolutionary divergence within SHFV that is roughly equivalent to the degree of divergence between other arterivirus species. The presence of two such highly divergent SHFV variants co-infecting a single individual represents a degree of within-host viral diversity that exceeds what has previously been reported for any arterivirus. These results expand our knowledge of the natural history and diversity of the arteriviruses and underscore the importance of wild primates as reservoirs for novel pathogens.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
14.9
作者：
Abascal F;Zardoya R;Telford MJ
通讯作者：
Telford MJ
影响因子：
3.7
作者：
GODENY, EK;CHEN, L;BRINTON, MA
通讯作者：
BRINTON, MA
影响因子：
0.9
作者：
Stadejek, T;Mittelholzer, C;Belák, S
通讯作者：
Belák, S
影响因子：
5.4
作者：
Goldberg, Tony L.;Sintasath, David M.;Switzer, William M.
通讯作者：
Switzer, William M.