Automated quantification of Candida albicans biofilm-related phenotypes reveals additive contributions to biofilm production.
Automated quantification of Candida albicans biofilm-related phenotypes reveals additive contributions to biofilm production.
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DOI：
10.1038/s41522-020-00149-5
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发表时间：
2020-10-09
影响因子：
9.2
通讯作者：
Anderson MZ
中科院分区：
文献类型：
--
作者：
Dunn MJ;Fillinger RJ;Anderson LM;Anderson MZ
生物膜是微生物细胞的有组织的群落，促进细菌和真菌物种之间的持久性。寄主相关的假丝酵母菌在组织表面和植入的装置上都会形成生物膜，有助于宿主的定植和疾病。在白色念珠菌中，生物膜是通过酵母菌附着在表面、侵入底物、形成气生菌丝突起和分泌细胞外基质而顺序形成的。对这些生物膜相关表型的测量仍然是高度定性的，而且往往是主观的。在这里，我们设计了一条信息学管道，用于量化念珠菌在固体琼脂介质上的丝状化、粘附性和侵袭性，并利用这种方法来确定这些成分表型对白色念珠菌生物被膜生产的重要性。对23株白色念珠菌临床分离株在三种介质和两种温度下的特征表明，在任何单一条件下分离株之间都有广泛的表型反应。培养基深刻地改变了这些菌株中所有与生物膜相关的表型，而温度对这些特征的影响最小。重要的是，在某些条件下，生物膜形成的程度与其成分表型的相加分数显著相关，实验上将每个成分的强度与生物膜质量联系起来。此外，基因组参考菌株SC5314在这些条件下的反应与所有其他菌株相比是一个极端的异常值，这表明它可能不能代表该物种。综上所述，开发一种高通量、无偏见的方法来量化念珠菌生物膜相关表型，将这些表型的可变性与生物膜的产生联系起来，并可以促进对这些关键过程的遗传解剖，从而在宿主中致病。
Biofilms are organized communities of microbial cells that promote persistence among bacterial and fungal species. Biofilm formation by host-associated Candida species of fungi occurs on both tissue surfaces and implanted devices, contributing to host colonization and disease. In C. albicans, biofilms are built sequentially by adherence of yeast to a surface, invasion into the substrate, the formation of aerial hyphal projections, and the secretion of extracellular matrix. Measurement of these biofilm-related phenotypes remains highly qualitative and often subjective. Here, we designed an informatics pipeline for quantifying filamentation, adhesion, and invasion of Candida species on solid agar media and utilized this approach to determine the importance of these component phenotypes to C. albicans biofilm production. Characterization of 23 C. albicans clinical isolates across three media and two temperatures revealed a wide range of phenotypic responses among isolates in any single condition. Media profoundly altered all biofilm-related phenotypes among these isolates, whereas temperature minimally impacted these traits. Importantly, the extent of biofilm formation correlated significantly with the additive score for its component phenotypes under some conditions, experimentally linking the strength of each component to biofilm mass. In addition, the response of the genome reference strain, SC5314, across these conditions was an extreme outlier compared to all other strains, suggesting it may not be representative of the species. Taken together, development of a high-throughput, unbiased approach to quantifying Candida biofilm-related phenotypes linked variability in these phenotypes to biofilm production and can facilitate genetic dissection of these critical processes to pathogenesis in the host.
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影响因子：
11.8
作者：
Horn, David L.;Neofytos, Dionissios;Webster, Karen M.
通讯作者：
Webster, Karen M.
影响因子：
3.4
作者：
Davis-Fields, Megan;Bakhtiari, Layla A.;Gordon, Vernita D.
通讯作者：
Gordon, Vernita D.
DOI：
10.1534/g3.111.000885
发表时间：
2011-12
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Abbey D;Hickman M;Gresham D;Berman J
通讯作者：
Berman J
DOI：
10.4161/trns.22281
发表时间：
2012-11
期刊：
Transcription
影响因子：
--
作者：
Fox EP;Nobile CJ
通讯作者：
Nobile CJ
影响因子：
3.1
作者：
Calera, JA;Zhao, XJ;Calderone, R
通讯作者：
Calderone, R