Subcellular localization of intercellular adhesion molecule-5 (telencephalin) in the visual cortex is not developmentally regulated in the absence of matrix metalloproteinase-9.
Subcellular localization of intercellular adhesion molecule-5 (telencephalin) in the visual cortex is not developmentally regulated in the absence of matrix metalloproteinase-9.
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DOI：
10.1002/cne.23440
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发表时间：
2014-02-15
影响因子：
2.5
通讯作者：
Majewska, Ania K.
中科院分区：
文献类型：
--
作者：
Kelly, Emily A.;Tremblay, Marie-Eve;Gahmberg, Carl G.;Tian, Li;Majewska, Ania K.
The telencephalon-associated intercellular adhesion molecule 5 (Telencephalin; ICAM-5) regulates dendritic morphology in the developing brain. In vitro studies have shown that ICAM-5 is predominantly found within dendrites and immature dendritic protrusions, with reduced expression in mushroom spines, suggesting that ICAM-5 downregulation is critical for the maturation of synaptic structures. However, developmental expression of ICAM-5 has not been explored in depth at the ultrastructural level in intact brain tissue. To investigate the ultrastructural localization of ICAM-5 with transmission electron microscopy, we performed immunoperoxidase histochemistry for ICAM-5 in mouse visual cortex at postnatal day (P)14, a period of intense synaptogenesis, and at P28, when synapses mature. We observed the expected ICAM-5 expression in dendritic protrusions and shafts at both P14 and P28. ICAM-5 expression in these dendritic protrusions decreased in prevalence with developmental age to become predominantly localized to dendritic shafts by P28. To further understand the relationship between ICAM-5 and the endopeptidase metalloproteinase-9 (MMP-9), which mediates ICAM-5 cleavage following glutamate activation during postnatal development, we also explored ICAM-5 expression in MMP-9 null animals. This analysis revealed a similar expression of ICAM-5 in dendritic elements at P14 and P28; however an increased prevalence of ICAM-5 was noted in dendritic protrusions at P28 in the MMP-9 null animals, indicating that in the absence of MMP-9, there is no developmental shift in ICAM-5 subcellular localization. Our ultrastructural observations shed light on possible functions mediated by ICAM-5 and their regulation by extracellular proteases.
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影响因子：
5.3
作者：
Furutani, Yutaka;Matsuno, Hitomi;Yoshihara, Yoshihiro
通讯作者：
Yoshihara, Yoshihiro
影响因子：
4.7
作者：
Bouvier, David;Corera, Amadou T.;Doucet, Guy
通讯作者：
Doucet, Guy
DOI：
10.1016/s0169-328x(98)00040-0
发表时间：
1998-06-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Ito, D;Imai, Y;Kohsaka, S
通讯作者：
Kohsaka, S
影响因子：
2.5
作者：
Mortillo, Steven;Elste, Alice;Ge, Yongchao;Patil, Shekhar B.;Hsiao, Kuangfu;Huntley, George W.;Davis, Ronald L.;Benson, Deanna L.
通讯作者：
Benson, Deanna L.
影响因子：
4.7
作者：
Conant, Katherine;Lonskaya, Irina;Lim, Seung T.
通讯作者：
Lim, Seung T.