Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies.
Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies.
复制标题
通过单克隆抗体酶联免疫吸附测定法调查日本不同地区的人类轮状病毒血清型。
DOI：
10.1093/infdis/160.1.44
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Mitsuaki Ohseto
中科院分区：
文献类型：
--
作者：
S. Urasawa;T. Urasawa;K. Taniguchi;F. Wakasugi;N. Kobayashi;Shunzo Chiba;N. Sakurada;M. Morita;O. Morita;Masayoshi Tokieda;H. Kawamoto;Y. Minekawa;Mitsuaki Ohseto
为了调查在日本流行的人轮状病毒血清型的相对频率，1986年11月至1988年3月在7个地区收集了562例轮状病毒胃肠炎患者的粪便标本，采用酶联免疫吸附试验（ELISA）与血清型1，2，3和4特异性单克隆抗体进行了检查。1986-1987年冬季，血清型1为主要血清型;然而，1987-1988年冬季，血清型1和2均频繁检出。结果显示了各地区各血清型的相对频率和同一地区各血清型流行的年度变化。通过用亚组I-和II-特异性单克隆抗体使用ELISA获得的轮状病毒的亚组特异性的结果证实了具有亚组I特异性的轮状病毒株是血清型2和具有亚组II特异性的轮状病毒株是血清型1、3或4的一般发现。还发现了具有亚组I和II特异性或无特异性的不常见菌株以及假定代表新血清型的菌株。
To investigate the relative frequency of individual human rotavirus serotypes prevailing in Japan, 562 stool specimens collected from patients with rotavirus gastroenteritis between November 1986 and March 1988 in seven districts were examined by an enzyme-linked immunosorbent assay (ELISA) with serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies. Serotype 1 was the predominant serotype in the winter of 1986-1987; however, both serotypes 1 and 2 were detected frequently in the winter of 1987-1988. The results showed the relative frequency of individual serotypes by locale and the yearly change in the prevalence of each serotype in the same area. The result of subgroup specificity of rotavirus obtained by using ELISA with subgroup I- and II-specific monoclonal antibodies confirmed the general finding that rotavirus strains having subgroup I specificity are serotype 2 and those having subgroup II specificity are either serotype 1, 3, or 4. Unusual strains having both subgroup I and II specificity or neither specificity and strains presumed to represent new serotypes were also found.
影响因子：
3.7
作者：
LIU, M;OFFIT, PA;ESTES, MK
通讯作者：
ESTES, MK