Investigating Membrane Interactions and Structures of CPPs.
Investigating Membrane Interactions and Structures of CPPs.
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研究 CPP 的膜相互作用和结构。
DOI：
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发表时间：
2015
影响因子：
--
通讯作者：
A. Gräslund
中科院分区：
文献类型：
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作者：
F. Madani;A. Gräslund
尽管对细胞穿透肽（CPP）进行了许多研究，但其细胞摄取和内体逃逸的机制尚未完全解决。当CPP与货物分子共价或非共价结合时，这甚至更不清楚。为了回答剩下的问题，我们需要结合不同的方法，模型系统和实验，因为没有一种方法可以给出所有问题的完整答案。CPP的生物物理学研究对CPP的分子作用机制研究有重要影响。在这一章中，我们提出了不同的膜模型系统，适合生物物理研究以及基本的实际方面的几个常见的生物物理方法和实验。这些方法包括荧光光谱法、圆二色光谱法和动态光散射法，并涉及肽-膜相互作用和囊泡模型膜渗漏。我们还描述了CPP-膜相互作用的生物物理研究的潜力和局限性，以及它们对我们理解CPP如何介导货物运输到活细胞的影响。
Despite many studies made on cell-penetrating peptides (CPPs), the mechanism of their cellular uptake and endosomal escape has not been completely resolved. This is even more unclear when the CPP is bound either covalently or non-covalently to the cargo molecules. To answer remaining questions, we require a combination of different methods, model systems, and experiments since there is no single method which could give a complete answer to all questions. Biophysical investigations of CPPs have a significant impact on CPP research considering their molecular mechanisms of action. In this chapter, we present different membrane model systems suitable for biophysical studies as well as the basic practical aspects underlying several common biophysical methods and experiments. The methods include fluorescence spectroscopy, circular dichroism spectroscopy, and dynamic light scattering and concern peptide-membrane interactions and vesicle model membrane leakage. We have also described the potential and limitations of biophysical studies on the CPP-membrane interactions and their impact on our understanding of how CPPs mediate the transport of cargoes into living cells.
用于染料释放研究的荧光素钠封装。
影响因子：
4.4
作者：
Niesman,MR;Khoobehi,B;Peyman,GA
通讯作者：
Peyman,GA
DOI：
10.1016/0005-2736(91)90295-j
发表时间：
1991-01-30
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MACDONALD, RC;MACDONALD, RI;HU, LR
通讯作者：
HU, LR