Tagmentation-based single-cell genomics.
Tagmentation-based single-cell genomics.
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DOI：
10.1101/gr.275223.121
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发表时间：
2021-10
期刊：
影响因子：
7
通讯作者：
Adey AC
中科院分区：
文献类型：
--
作者：
Adey AC
自从最初描述基于转座酶的方法来制备高通量测序文库或“标签化”以来，已经过去了10多年，其中使用高度活跃的转座酶来同时片段化靶DNA并附加通用衔接子序列。标签化有效地取代了传统工作流程中的一系列处理步骤，只需一个反应。简单性加上标签片段化的高效率，使其成为测序文库构建的有利手段，并促进了多种适应性以测定各种分子特性。近年来，这一直集中在单细胞领域，已经开发了一系列基于标记的检测方法，覆盖了监管领域的大量领域。到目前为止，已经有一些优秀的评论单细胞技术结构周围的分子特性，可以分析。这篇综述是围绕标签片段化的核心组成部分和性质以及它们如何使创新的分子工具的开发能够探测单细胞的调控景观。此外，没有讨论细胞通量的粒度细节或由广泛的单个技术列表提供的数据的丰富性。这些指标变化迅速，具有高度的样本特异性，最好留给那些在严格控制的框架内直接比较检测技术的研究。这次审查的希望是，在这些检测平台的每个阶段的分子技术的多样性，新的想法可能会出现其他人追求，将进一步推进单细胞基因组学领域。
It has been just over 10 years since the initial description of transposase-based methods to prepare high-throughput sequencing libraries, or “tagmentation,” in which a hyperactive transposase is used to simultaneously fragment target DNA and append universal adapter sequences. Tagmentation effectively replaced a series of processing steps in traditional workflows with one single reaction. It is the simplicity, coupled with the high efficiency of tagmentation, that has made it a favored means of sequencing library construction and fueled a diverse range of adaptations to assay a variety of molecular properties. In recent years, this has been centered in the single-cell space with a catalog of tagmentation-based assays that have been developed, covering a substantial swath of the regulatory landscape. To date, there have been a number of excellent reviews on single-cell technologies structured around the molecular properties that can be profiled. This review is instead framed around the central components and properties of tagmentation and how they have enabled the development of innovative molecular tools to probe the regulatory landscape of single cells. Furthermore, the granular specifics on cell throughput or richness of data provided by the extensive list of individual technologies are not discussed. Such metrics are rapidly changing and highly sample specific and are better left to studies that directly compare technologies for assays against one another in a rigorously controlled framework. The hope for this review is that, in laying out the diversity of molecular techniques at each stage of these assay platforms, new ideas may arise for others to pursue that will further advance the field of single-cell genomics.
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DOI：
10.1073/pnas.1919800117
发表时间：
2020-02-11
影响因子：
11.1
作者：
Di, Lin;Fu, Yusi;Wang, Jianbin
通讯作者：
Wang, Jianbin
DOI：
10.1126/science.aau0730
发表时间：
2018-09-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cao J;Cusanovich DA;Ramani V;Aghamirzaie D;Pliner HA;Hill AJ;Daza RM;McFaline-Figueroa JL;Packer JS;Christiansen L;Steemers FJ;Adey AC;Trapnell C;Shendure J
通讯作者：
Shendure J
影响因子：
64.5
作者：
Dixit, Atray;Pamas, Oren;Li, Biyu;Chen, Jenny;Fulco, Charles P.;Jerby-Amon, Livnat;Marjanovic, Nemanja D.;Dionne, Danielle;Burks, Tyler;Raychowdhury, Raktima;Adamson, Britt;Norman, Thomas M.;Lander, Eric S.;Weissman, Jonathan S.;Friedman, Nir;Regev, Aviv
通讯作者：
Regev, Aviv
单细胞染色质可及性揭示了调节变化的原理。
影响因子：
64.8
作者：
Buenrostro JD;Wu B;Litzenburger UM;Ruff D;Gonzales ML;Snyder MP;Chang HY;Greenleaf WJ
通讯作者：
Greenleaf WJ
影响因子：
56.9
作者：
Cao, Junyue;Packer, Jonathan S.;Shendure, Jay
通讯作者：
Shendure, Jay