TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat.
TaNAC020 homoeologous genes are associated with higher thousand kernel weight and kernel length in Chinese wheat.
复制标题
TaNAC020同源基因与中国小麦较高的千粒重和粒长相关。
DOI：
10.3389/fgene.2022.956921
复制
发表时间：
2022
影响因子：
3.7
通讯作者：
Zhang, Xueyong
中科院分区：
文献类型：
--
作者：
Majeed, Uzma;Hou, Jian;Hao, Chenyang;Zhang, Xueyong
NAC蛋白是最大的植物特异性转录因子家族之一，在植物生长发育中起着重要作用。本研究从小麦（Triticum aestivum L.）中分离到3个TaNAC 020同源异型基因，分别位于染色体7A、7 B和7 D上。TaNAC 020主要在发育中的谷粒中表达。转TaNAC 020-B基因水稻株系的淀粉密度和直链淀粉含量均高于野生型（WT）。序列多态性研究表明，在TaNAC 020-A/B中有7个和8个SNP，分别形成3个和2个单倍型。在TaNAC 020-D中未发现序列多态性。关联分析表明，TaNAC 020-A和TaNAC 020-B的HAP-2是具有较高千粒重和长度的有利单倍型。地理分布和等位基因频率表明，我们所青睐的单倍型在中国经历了强烈的选择，同样，在小麦多倍体化过程中，TaNAC 020 s的多样性增加。研究结果表明，TaNAC 020 s对小麦籽粒淀粉合成和积累有积极影响，是控制籽粒大小和粒数的关键调控因子之一，在小麦育种中具有潜在的应用价值。本研究开发的分子标记有助于小麦遗传改良和种质创新的分子标记辅助选择。
NAC proteins constitute one of the largest plant-specific transcription factor (TF) families and play significant roles in plant growth and development. In the present study, three TaNAC020 homoeologous genes located on chromosomes 7A, 7B, and 7D were isolated from wheat (Triticum aestivum L.). TaNAC020s were predominantly expressed in developing grains. The developed transgenic rice lines for TaNAC020-B showed higher starch density and lower amylose contents than those of the wild type (WT). Sequence polymorphism studies showed seven and eight SNPs in TaNAC020-A/B, making three and two haplotypes, respectively. No sequence polymorphism was identified in TaNAC020-D. Association analysis revealed that HAP-2 of TaNAC020-A and TaNAC020-B was the favored haplotype for higher thousand kernel weight and length. Geographic distribution and allelic frequency showed that our favored haplotype experienced strong selection in China, and likewise, diversity increased in TaNAC020s during wheat polyploidization. The results obtained in this study demonstrate that TaNAC020s positively influence starch synthesis and accumulation and are one of the key regulators of the kernel (seed) size and kernel number and have the potential for utilization in wheat breeding to improve grain yield. Molecular markers developed in this study stand instrumental in marker-assisted selection for genetic improvement and germplasm enhancement in wheat.
登录
查看更多内容
中国小麦品种NAM-B1基因等位基因分布及变异分析
影响因子：
4.8
作者：
Chen Xue-yan;Song Guo-qi;Ji Wan-quan
通讯作者：
Ji Wan-quan
影响因子：
5.9
作者：
Brinton J;Ramirez-Gonzalez RH;Simmonds J;Wingen L;Orford S;Griffiths S;10 Wheat Genome Project;Haberer G;Spannagl M;Walkowiak S;Pozniak C;Uauy C
通讯作者：
Uauy C
DOI：
10.1073/pnas.252763199
发表时间：
2003-03-04
影响因子：
11.1
作者：
Peng, JH;Ronin, Y;Korol, A
通讯作者：
Korol, A
影响因子：
5.4
作者：
Rasheed, Awais;Wen, Weie;He, Zhonghu
通讯作者：
He, Zhonghu
与产量贡献性状相关的小麦蛋白激酶基因TaSnRK2.9-5A
影响因子：
5.4
作者：
Rehman, Shoaib Ur;Wang, Jingyi;Jing, Ruilian
通讯作者：
Jing, Ruilian