The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect.
The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect.
复制标题
载有间充质干细胞 (MSC)、骨形态发生蛋白 2 和富血小板血浆 (PRP) 的明胶 β-磷酸三钙海绵对马关节软骨缺损的作用。
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
N. Sasaki
中科院分区：
文献类型：
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作者：
N. Tsuzuki;Jong;Kazutaka Yamada;S. Haneda;H. Furuoka;Y. Tabata;N. Sasaki
我们评估了明胶β-磷酸三钙（β-TCP）海绵植入实验诱导的骨软骨缺损的疗效，该海绵载有间充质干细胞（MSC）、骨形态发生蛋白-2（BMP-2）和富血小板血浆（PRP）。在7匹纯种马的双侧内侧股骨髁上钻一个直径和深度为10 mm的孔，并将加载的海绵（治疗）或盐水灌注的β-TCP海绵（对照）插入每个孔中。16周后，通过计算机断层扫描、宏观分析和组织学分析检查缺损。治疗腿的中位软骨下骨密度和关节愈合的宏观分项评分显著较高（P < 0.05）。尽管组间总组织学评分无显著差异，但在治疗组中观察到更广泛区域的透明软骨组织。在病变部位插入BMP-2、MSC和PRP浸渍的β-TCP海绵可以增强马关节愈合。
We evaluated the curative efficacy of a gelatin β-tricalcium phosphate (β-TCP) sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2 (BMP-2), and platelet-rich plasma (PRP) by insertion into an experimentally induced osteochondral defect. A hole of 10 mm diameter and depth was drilled in the bilateral medial femoral condyles of 7 thoroughbred horses, and into each either a loaded sponge (treatment) or a saline-infused β-TCP sponge (control) was inserted. After 16 weeks, defects were examined by computed tomography, macroscopic analyses, and histological analyses. The median subchondral bone density and macroscopic subscores for joint healing were significantly higher in the treatment legs (P < 0.05). Although there was no significant difference in total histological scores between groups, hyaline cartilaginous tissue was observed across a wider area in the treatment group. Equine joint healing can be enhanced by inserting a BMP-2-, MSC-, and PRP-impregnated β-TCP sponge at the lesion site.
影响因子：
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作者：
Neu, C. P.;Reddi, A. H.;Komvopoulos, K.;Schmid, T. M.;Di Cesare, P. E.
通讯作者：
Di Cesare, P. E.
影响因子：
4.1
作者：
Bian, Liming;Zhai, David Y.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.