Increased friction coefficient and superficial zone protein expression in patients with advanced osteoarthritis.
Increased friction coefficient and superficial zone protein expression in patients with advanced osteoarthritis.
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DOI：
10.1002/art.27577
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发表时间：
2010-09
影响因子：
--
通讯作者：
Di Cesare, P. E.
中科院分区：
文献类型：
--
作者：
Neu, C. P.;Reddi, A. H.;Komvopoulos, K.;Schmid, T. M.;Di Cesare, P. E.
定量测定晚期骨关节炎（OA）患者关节软骨和滑液中表浅区蛋白（SZP）的浓度，并进一步将SZP含量与摩擦系数、OA严重程度和炎性细胞因子水平相关联。关节软骨和滑液的样品从接受择期全膝关节置换手术的患者中获得。从捐赠的身体计划和组织库来源获得额外的正常样本。通过酶联免疫吸附测定和免疫组织化学可视化从股骨髁获得的软骨中的区域SZP表达进行定量。摩擦系数的测量是从软骨塞滑动边界润滑制度。使用组织化学分析对OA严重程度进行分级。酶联免疫吸附法测定关节液中SZP和炎性细胞因子的浓度。SZP在膝关节软骨中的定位模式被确定，其中承重区域表现出高SZP表达。SZP模式与摩擦系数和OA严重程度相关;然而，无论OA严重程度如何，在关节面的所有样本中均观察到SZP表达。SZP表达和关节液抽吸量在OA患者中高于正常对照组。部分患者滑液中细胞因子表达升高。结果揭示了机械力化学耦合，其中物理力调节OA的严重程度和关节润滑。这项研究的结果还表明，SZP可能是无效的，在减少关节摩擦的边界润滑制度在先进的OA阶段，其他机制可能占主导地位的摩擦学行为。
To quantify the concentration of superficial zone protein (SZP) in articular cartilage and synovial fluid of patients with advanced osteoarthritis (OA), and to further correlate the SZP content with the friction coefficient, OA severity, and levels of inflammatory cytokines. Samples of articular cartilage and synovial fluid were obtained from patients undergoing elective total knee replacement surgery. Additional normal samples were obtained from donated body program and tissue bank sources. Regional SZP expression in cartilage obtained from the femoral condyles was quantified by enzyme-linked immunosorbant assay and visualized by immunohistochemistry. Friction coefficient measurements were obtained from cartilage plugs slid in the boundary lubrication regime. OA severity was graded using histochemical analyses. The concentration of SZP and inflammatory cytokines in synovial fluid were determined by enzyme-linked immunosorbant assays. A pattern of SZP localization in knee cartilage was identified, with load-bearing regions exhibiting high SZP expression. SZP patterns correlated to friction coefficient and OA severity; however SZP expression was observed in all samples at the articular surface, regardless of OA severity. SZP expression and aspirate volume of synovial fluid were higher in OA patients compared to normal controls. Expressions of cytokines were elevated in the synovial fluid of some patients. The results reveal a mechano-chemical coupling in which physical forces regulate OA severity and joint lubrication. The findings of this study also suggest that SZP may be ineffective in reducing joint friction in the boundary lubrication regime at an advanced OA stage where other mechanisms may dominate the observed tribological behavior.
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影响因子：
--
作者：
Neu, Corey P.;Khalafi, Afshin;Reddi, A. Hari
通讯作者：
Reddi, A. Hari
影响因子：
3.9
作者：
SCHUMACHER, BL;BLOCK, JA;KUETTNER, KE
通讯作者：
KUETTNER, KE
影响因子：
2.8
作者：
DuRaine, Grayson;Neu, Corey P.;Reddi, A. Hari
通讯作者：
Reddi, A. Hari
影响因子：
56.9
作者：
KRUPINSKI, J;COUSSEN, F;GILMAN, AG
通讯作者：
GILMAN, AG
影响因子：
--
作者：
Jones, Aled R. C.;Chen, Shuodan;Flannery, Carl R.
通讯作者：
Flannery, Carl R.