Dielectrophoresis-based liposome delivery to a planar lipid membrane for efficient membrane protein reconstitution
Dielectrophoresis-based liposome delivery to a planar lipid membrane for efficient membrane protein reconstitution
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基于介电泳的脂质体递送至平面脂质膜以实现有效的膜蛋白重建
DOI：
10.1109/memsys.2010.5442360
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
S. Takeuchi
中科院分区：
文献类型：
--
作者：
T. Osaki;S. Takeuchi
这项工作提出了一种利用介电泳（DEP）力将脂质体中的膜蛋白有效掺入平面双层脂质膜的策略。该装置由一个腔室和一个通道组成，通道之间由微米大小的孔径隔开，该孔径悬挂着平面双层膜。在膜上施加交流电场，DEP 力集中在双层区域，因此吸引脂质体。我们还观察到在 DEP 作用下脂质体自动融合到脂质膜中。这种基于 DEP 的融合方法将非常适用于膜蛋白研究。
This work presents a strategy that realizes efficient incorporation of membrane proteins in liposomes into a planar bilayer lipid membrane by using dielectrophoretic (DEP) force. The device consists of a chamber and a channel separated by a µm-sized aperture that suspends a planar bilayer membrane. Applying an AC electric field across the membrane, the DEP force concentrates at the bilayer area therefore attracts the liposomes. We also observed fusion of liposomes autonomously occurs into the lipid membrane under the DEP effect. This DEP-based fusion method will be highly applicable to membrane protein study.
影响因子：
5.8
作者：
Estes, DJ;Mayer, M
通讯作者：
Mayer, M