Transfection of shRNA-encoding Minivector DNA of a few hundred base pairs to regulate gene expression in lymphoma cells.
Transfection of shRNA-encoding Minivector DNA of a few hundred base pairs to regulate gene expression in lymphoma cells.
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这项工作说明了微型载体DNA，一种非病毒的，超螺旋的基因治疗载体纳入短发夹RNA从H1启动子的效用。微载体DNA比质粒DNA和小干扰RNA（siRNA）优越上级，因为它具有改善的生物稳定性，同时保持高的细胞转染效率和基因沉默能力。微载体DNA在人血清中稳定超过48小时，而siRNA和质粒分别仅为0.5和2小时。虽然所有三种核酸在容易转染的粘附成纤维细胞中表现出相似的转染效率，但只有Minivector DNA和siRNA能够转染难以转染的悬浮淋巴瘤细胞。微载体DNA和siRNA能够沉默编码间变性淋巴瘤激酶的基因，该基因是人类间变性大细胞淋巴瘤的关键致病因子，并且这种沉默引起淋巴瘤细胞的抑制。基于这些结果，微载体DNA是一种有前途的新的基因治疗工具。
This work illustrates the utility of Minivector DNA, a non-viral, supercoiled gene therapy vector incorporating short hairpin RNA from an H1 promoter. Minivector DNA is superior to both plasmid DNA and small interfering RNA (siRNA) in that it has improved biostability while maintaining high cell transfection efficiency and gene silencing capacity. Minivector DNAs were stable for over 48 h in human serum, as compared with only 0.5 and 2 h for siRNA and plasmid, respectively. Although all three nucleic acids exhibited similar transfection efficiencies in easily transfected adhesion fibroblasts cells, only Minivector DNAs and siRNA were capable of transfecting difficult-to-transfect suspension lymphoma cells. Minivector DNA and siRNA were capable of silencing the gene encoding anaplastic lymphoma kinase, a key pathogenic factor of human anaplastic large cell lymphoma, and this silencing caused inhibition of the lymphoma cells. Based on these results, Minivector DNAs are a promising new gene therapy tool.
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影响因子：
14.9
作者：
Ma, Hoi Tang;On, Kin Fan;Tsang, Yiu Huen;Poon, Randy Y. C.
通讯作者：
Poon, Randy Y. C.
影响因子：
5.1
作者：
Chen, ZY;He, CY;Kay, MA
通讯作者：
Kay, MA
影响因子：
14.9
作者：
Kreiss, P;Cameron, B;Pitard, B
通讯作者：
Pitard, B
影响因子：
11.4
作者：
Shi, Y
通讯作者：
Shi, Y
影响因子：
20.3
作者：
Piva, R;Chiarle, R;Inghirami, G
通讯作者：
Inghirami, G