Plasma membrane topography governs the 3D dynamic localization of IgM B cell antigen receptor clusters.
Plasma membrane topography governs the 3D dynamic localization of IgM B cell antigen receptor clusters.
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DOI：
10.15252/embj.2022112030
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发表时间：
2023-02-15
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影响因子：
--
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B淋巴细胞通过不同类型的B细胞抗原受体（BCR）识别细菌或病毒抗原。称为微绒毛的突出结构覆盖淋巴细胞表面，并且被认为在筛选抗原承载表面中执行感觉功能。在这里，我们使用点阵光片显微镜结合定制的定制4D图像分析来研究拉莫斯伯基特淋巴瘤细胞系的B细胞的细胞表面形貌和IgM‐BCR的时空组织。发现拉莫斯B-细胞表面形成桥接单个微绒毛的隆起脊的动态网络。一部分膜定位的IgM‐BCR被发现成簇，主要与脊和微绒毛相关。动态脊网组织和IgM-BCR簇的移动性是相关的，两者都受Arp 2/3复合物活性的控制。我们的研究结果表明，细胞表面的动态地形特征支配IgM‐BCR簇的定位和转运，以促进B细胞的抗原筛选。伯基特淋巴瘤拉莫斯B细胞系的高分辨率显微镜显示，细胞表面的动态地形特征支配肌动蛋白和微管细胞骨架上游的B细胞受体的质膜定位。
B lymphocytes recognize bacterial or viral antigens via different classes of the B cell antigen receptor (BCR). Protrusive structures termed microvilli cover lymphocyte surfaces, and are thought to perform sensory functions in screening antigen‐bearing surfaces. Here, we have used lattice light‐sheet microscopy in combination with tailored custom‐built 4D image analysis to study the cell‐surface topography of B cells of the Ramos Burkitt's Lymphoma line and the spatiotemporal organization of the IgM‐BCR. Ramos B‐cell surfaces were found to form dynamic networks of elevated ridges bridging individual microvilli. A fraction of membrane‐localized IgM‐BCR was found in clusters, which were mainly associated with the ridges and the microvilli. The dynamic ridge‐network organization and the IgM‐BCR cluster mobility were linked, and both were controlled by Arp2/3 complex activity. Our results suggest that dynamic topographical features of the cell surface govern the localization and transport of IgM‐BCR clusters to facilitate antigen screening by B cells. High‐resolution microscopy of the Burkitt's lymphoma Ramos B cell line reveals that dynamic topographical features of the cell surface govern plasma membrane localization of the B cell receptor upstream of actin and microtubule cytoskeleton.
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影响因子：
--
作者：
de Kreuk BJ;Hordijk PL
通讯作者：
Hordijk PL
影响因子：
5.9
作者：
Franke C;Chum T;Kvíčalová Z;Glatzová D;Gentsch GJ;Rodriguez A;Helmerich DA;Herdly L;Mavila H;Frank O;Brdička T;van de Linde S;Cebecauer M
通讯作者：
Cebecauer M
影响因子：
16.6
作者：
Jung Y;Wen L;Altman A;Ley K
通讯作者：
Ley K
影响因子：
9.2
作者：
Fricke, Robert;Gohl, Christina;Bogdan, Sven
通讯作者：
Bogdan, Sven
DOI：
10.1109/tvcg.2015.2446493
发表时间：
2016-06-01
影响因子：
5.2
作者：
Jerman, Tim;Pernus, Franjo;Spiclin, Ziga
通讯作者：
Spiclin, Ziga