Selective hemoglobin inhibition of endothelium-dependent vasodilation of rabbit basilar artery.
Selective hemoglobin inhibition of endothelium-dependent vasodilation of rabbit basilar artery.
复制标题
选择性血红蛋白抑制兔基底动脉内皮依赖性血管舒张。
作者：
S. Fujiwara;N. Kassell;T. Sasaki;T. Nakagomi;R. Lehman
用等长张力记录法观察血红蛋白对兔离体基底动脉内皮依赖性血管舒张的影响。乙酰胆碱（ACh） (10(-7) - (10(-4) M)可引起10(-6)M血清素收缩的离体兔基底动脉的剂量依赖性血管扩张。这种血管舒张作用在去内皮后消失。乙酰胆碱刺激引起的兔基底动脉血管舒张被认为是严格依赖于内皮细胞的。血红蛋白(10（-7)- 10(-5)M）在一定剂量下抑制乙酰胆碱诱导的内皮依赖性血管舒张。三磷酸腺苷(ATP, 10（-7) - 10(-4) M）也能放松已被10(-6)M血清素收缩的离体兔基底动脉。这种血管扩张作用被腺苷拮抗剂8-苯基茶碱（8-PT）轻微抑制，并通过去除内皮显着减弱。这种ATP诱导的血管舒张被认为是由ATP本身（内皮依赖性）和ATP降解产物（内皮非依赖性）如单磷酸腺苷或腺苷组成的。血红蛋白明显抑制atp诱导的血管舒张，但仍有较小的血管舒张，被8-PT阻断。去除内皮不影响罂粟碱诱导的血管舒张，血红蛋白不抑制罂粟碱诱导的血管舒张。提示兔基底动脉具有乙酰胆碱和ATP诱导的内皮依赖性血管舒张机制，且血红蛋白选择性阻断了内皮依赖性血管舒张。这一发现可能与蛛网膜下腔出血后脑血管痉挛的发病机制有关：溶红细胞释放的血红蛋白可能抑制脑动脉内皮依赖性血管舒张；此外，蛛网膜下腔出血后的内皮变性可能损害大脑动脉平滑肌细胞的血管舒张机制。
The effect of hemoglobin on endothelium-dependent vasodilation of the isolated rabbit basilar artery was examined using an isometric tension recording method. Acetylcholine (ACh) (10(-7) - (10(-4) M) evoked a dose-dependent vasodilation of isolated rabbit basilar artery previously contracted by 10(-6) M serotonin. This vasodilating action disappeared after removal of the endothelium. The ACh-induced vasodilation of rabbit basilar artery is thought to be strictly endothelium-dependent. Hemoglobin (10(-7) - 10(-5) M) inhibited this ACh-induced endothelium-dependent vasodilation conditional upon the dose. Adenosine triphosphate (ATP, 10(-7) - 10(-4) M) also relaxed isolated rabbit basilar artery already contracted by 10(-6) M serotonin. This vasodilating action was slightly inhibited by adenosine antagonist, 8-phenyltheophylline (8-PT), and markedly attenuated by removal of the endothelium. This ATP-induced vasodilation is thought to be composed of ATP itself (endothelium-dependent) and ATP degradation products (endothelium-independent) such as adenosine monophosphate or adenosine. Hemoglobin markedly inhibited ATP-induced vasodilation, but there still remained a small vasodilation, which was blocked by 8-PT. Papaverine-induced vasodilation was not affected by removal of the endothelium, and hemoglobin did not inhibit the papaverine-induced vasodilation. These results suggest that rabbit basilar artery has endothelium-dependent vasodilating mechanisms induced by ACh and ATP, and that hemoglobin selectively blocks the endothelium-dependent vasodilation. This finding may relate to the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage: there is a possibility that the presence of hemoglobin released from lysed erythrocytes inhibits the endothelium-dependent vasodilation of cerebral arteries; furthermore, the endothelial degeneration following subarachnoid hemorrhage may impair the vasodilating mechanisms of cerebral artery smooth-muscle cells.
DOI：
--
发表时间：
1985-03
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
通讯作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
影响因子：
3
作者：
FURCHGOTT, RF;CHERRY, PD;JOTHIANANDAN, D
通讯作者：
JOTHIANANDAN, D
DOI：
10.1073/pnas.79.6.2106
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHERRY, PD;FURCHGOTT, RF;JOTHIANANDAN, D
通讯作者：
JOTHIANANDAN, D