Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton.
Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton.
复制标题
陆地棉晚期胚胎发生丰富（LEA）蛋白家族的特征及其在干旱胁迫耐受性中的作用
DOI：
10.1186/s12863-017-0596-1
复制
发表时间：
2018-01-15
期刊：
影响因子：
2.9
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Magwanga RO;Lu P;Kirungu JN;Lu H;Wang X;Cai X;Zhou Z;Zhang Z;Salih H;Wang K;Liu F
晚期胚胎发生丰富蛋白(LEA)是一大类亲水性蛋白，在植物对干旱和其他非生物胁迫的耐受性中起主要作用。对LEA蛋白家族的深入研究和鉴定已经在其他植物中进行，但在陆地棉中还没有。本研究的主要目的是对陆地棉后期胚胎发生丰富(LEA)蛋白家族进行特征分析，并进行基因表达分析，以确定其在陆地棉耐旱胁迫中的潜在作用。棉花是纺织工业的支柱产业，也是全球许多国家的经济作物。结果在这项工作中，共鉴定出242、136和142个LEA基因。Hirsuum.arboreum和G.分别为拉蒙迪。根据它们的保守结构域和系统发育树分析，将所鉴定的基因分为8个组。LeA-2的含量最高，这可能与其疏水性有关。陆地棉LEA基因内含子较少，分布于所有染色体。大多数重复的LEA基因是节段性的。Syntenic分析表明，LEA基因有较大比例的保守性。节段性基因复制在LEAgene的扩增中起关键作用。63个miRNAs针对89个基因，如miR164、ghr-miR394等。基因本体论分析表明，LEA基因参与了植物的脱水和防御反应。启动子中几乎所有的LEA基因都含有ABRE、MBS、W-Box和TAC元件，这些元件在功能上都与干旱胁迫和其他胁迫反应有关。大多数LEA基因都参与了分泌途径。表达谱分析表明，大多数LEA基因在抗旱性较强的棉花品种中高表达，而在抗旱性较弱的陆地棉中表达较低。耐旱品种在干旱胁迫下对基因的调控能力强于对干旱敏感的陆地棉品种。结论本研究为陆地棉、陆地棉LEA基因及其在干旱胁迫下的可能功能提供了较为全面的信息。
BackgroundLate embryogenesis abundant (LEA) proteins are large groups of hydrophilic proteins with major role in drought and other abiotic stresses tolerance in plants. In-depth study and characterization of LEA protein families have been carried out in other plants, but not in upland cotton. The main aim of this research work was to characterize the late embryogenesis abundant (LEA) protein families and to carry out gene expression analysis to determine their potential role in drought stress tolerance in upland cotton. Increased cotton production in the face of declining precipitation and availability of fresh water for agriculture use is the focus for breeders, cotton being the backbone of textile industries and a cash crop for many countries globally.ResultsIn this work, a total of 242, 136 and 142LEAgenes were identified inG. hirsutum,G. arboreumandG. raimondiirespectively. The identified genes were classified into eight groups based on their conserved domain and phylogenetic tree analysis. LEA 2 were the most abundant, this could be attributed to their hydrophobic character. Upland cottonLEAgenes have fewer introns and are distributed in all chromosomes. Majority of the duplicatedLEAgenes were segmental. Syntenic analysis showed that greater percentages ofLEAgenes are conserved. Segmental gene duplication played a key role in the expansion ofLEAgenes. Sixty three miRNAs were found to target 89 genes, such as miR164, ghr-miR394 among others. Gene ontology analysis revealed thatLEAgenes are involved in desiccation and defense responses. Almost all theLEAgenes in their promoters contained ABRE, MBS, W-Box and TAC-elements, functionally known to be involved in drought stress and other stress responses. Majority of theLEAgenes were involved in secretory pathways. Expression profile analysis indicated that most of theLEAgenes were highly expressed in drought tolerant cultivarsGossypium tomentosumas opposed to drought susceptible,G. hirsutum. The tolerant genotypes have a greater ability to modulate genes under drought stress than the more susceptible upland cotton cultivars.ConclusionThe finding provides comprehensive information onLEAgenes in upland cotton,G. hirsutumand possible function in plants under drought stress.
登录
查看更多内容
影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
2.1
作者：
Du, Dongliang;Hao, Ruijie;Zhang, Qixiang
通讯作者：
Zhang, Qixiang
影响因子：
4.3
作者：
Cao, Jun;Li, Xiang
通讯作者：
Li, Xiang
DOI：
10.1007/978-3-662-47901-8_10
发表时间：
2015-01-01
期刊：
BRASSICA RAPA GENOME
影响因子：
--
作者：
Cheng, Feng;Wu, Jian;Wang, Xiaowu
通讯作者：
Wang, Xiaowu
影响因子：
5.6
作者：
Battaglia M;Covarrubias AA
通讯作者：
Covarrubias AA