Evolutionary convergence and divergence in archaeal chromosomal proteins and Chromo-like domains from bacteria and eukaryotes.
Evolutionary convergence and divergence in archaeal chromosomal proteins and Chromo-like domains from bacteria and eukaryotes.
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DOI：
10.1038/s41598-018-24467-z
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发表时间：
2018-04-18
影响因子：
4.6
通讯作者：
Aravind L
中科院分区：
文献类型：
--
作者：
Kaur G;Iyer LM;Subramanian S;Aravind L
类染色体超家族的SH 3折叠β桶结构域识别真核生物蛋白质中的表观遗传标记。它们的来源已经被放置在古细菌中，基于与染色质压缩Sul 7 d和Cren 7蛋白的表观结构相似性，或者基于序列同源物的存在在细菌中。使用序列和结构的证据，我们建立了古细菌Cren 7/Sul 7蛋白出现从锌带（ZnR）的祖先。此外，我们表明，祖先真核细胞的类染色质结构域从细菌的版本，可能获得早期的内共生，已经拥有了一个芳香族的笼子，用于识别修饰的氨基。这些细菌版本是分泌的SH 3-折叠结构域的辐射的一部分，其在肽聚糖或细胞外基质中的肽识别的背景下产生了色样结构域和经典的SH 3结构域。这表明Cren 7/Sul 7通过失去金属螯合作用和获得更强的疏水相互作用从ZnR前体收敛到“SH 3”样状态;它不太可能参与了类色域的进化。我们发现，古生菌拥有几个Cren 7/Sul 7相关蛋白与完整的锌螯合配体，我们预测发挥以前未研究的作用，在细胞分裂过程中的染色体分离的PRC桶和CdvA结构域蛋白。
SH3-fold-β-barrel domains of the chromo-like superfamily recognize epigenetic marks in eukaryotic proteins. Their provenance has been placed either in archaea, based on apparent structural similarity to chromatin-compacting Sul7d and Cren7 proteins, or in bacteria based on the presence of sequence homologs. Using sequence and structural evidence we establish that the archaeal Cren7/Sul7 proteins emerged from a zinc ribbon (ZnR) ancestor. Further, we show that the ancestral eukaryotic chromo-like domains evolved from bacterial versions, likely acquired from early endosymbioses, which already possessed an aromatic cage for recognition of modified amino-groups. These bacterial versions are part of a radiation of secreted SH3-fold domains, which spawned both chromo-like domains and classical SH3 domains in the context of peptide-recognition in the peptidoglycan or the extracellular matrix. This establishes that Cren7/Sul7 converged to a “SH3”-like state from a ZnR precursor via the loss of metal-chelation and acquisition of stronger hydrophobic interactions; it is unlikely to have participated in the evolution of the chromo-like domains. We show that archaea possess several Cren7/Sul7-related proteins with intact Zn-chelating ligands, which we predict to play previously unstudied roles in chromosome segregation during cell-division comparable to the PRC barrel and CdvA domain proteins.
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影响因子：
7.3
作者：
Burroughs, Alexander Maxwell;Ando, Yoshinari;Aravind, L.
通讯作者：
Aravind, L.
影响因子：
12.3
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影响因子：
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作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
2.9
作者：
Cheng H;Liao Y;Schaeffer RD;Grishin NV
通讯作者：
Grishin NV
影响因子：
2.9
作者：
Amero, Carlos D.;Boomershine, William P.;Xu, Yiren;Foster, Mark
通讯作者：
Foster, Mark