A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis.
A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis.
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小麦 PI4K 基因其产物具有苏氨酸自磷酸化活性，赋予拟南芥耐旱和耐盐能力
DOI：
10.1093/jxb/ert133
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发表时间：
2013-07
影响因子：
6.9
通讯作者：
Ma YZ
中科院分区：
文献类型：
--
作者：
Liu P;Xu ZS;Pan-Pan L;Hu D;Chen M;Li LC;Ma YZ
肌醇磷脂参与调节细胞膜上信号蛋白的募集和活性。磷脂酰肌醇(PI)4-激酶(PI4Ks)产生磷脂酰肌醇(PI4Ks)，是调节磷脂酰肌醇的前体。以前还没有关于植物中非生物胁迫反应的II型PI4K研究的报道。通过对干旱处理小麦(Triticum Aestivum)进行新的转录组测序，获得了一个胁迫诱导的II型PI4K基因，命名为TaPI4KIIγ。TaPI4KIIγ定位于细胞膜上，可进行苏氨酸自磷酸化，但没有检测到脂蛋白激酶活性。TaPI4KIIγ与小麦泛素融合降解蛋白(TaUDF1)的相互作用表明，TaPI4KII可能被该酶体系所降解。TaPI4KIIγ的过表达表明，它能提高种子萌发和幼苗生长过程中的耐旱性和盐逆性。γ7突变体是拟南芥中TaPI4KIIγ的同源基因，该突变体对盐、聚乙二醇和脱落酸敏感，TaPI4KIIγ在γ7突变体中的过表达补偿了胁迫敏感性。TaPI4KIIγ促进了拟南芥的根生长，提示TaPI4KIIγ可能通过促进根的生长来增强抗逆性。TaPI4KIIγ的过表达导致胁迫相关基因的表达水平发生变化，并导致几个生理性状的变化，从而使植物对胁迫更耐受。这些结果为过表达TaPI4KIIγ可以提高抗旱性和耐盐性提供了证据。
Phosphoinositides are involved in regulation of recruitment and activity of signalling proteins in cell membranes. Phosphatidylinositol (PI) 4-kinases (PI4Ks) generate PI4-phosphate the precursor of regulatory phosphoinositides. No type II PI4K research on the abiotic stress response has previously been reported in plants. A stress-inducible type II PI4K gene, named TaPI4KIIγ, was obtained by de novo transcriptome sequencing of drought-treated wheat (Triticum aestivum). TaPI4KIIγ, localized on the plasma membrane, underwent threonine autophosphorylation, but had no detectable lipid kinase activity. Interaction of TaPI4KIIγ with wheat ubiquitin fusion degradation protein (TaUDF1) indicated that it might be hydrolysed by the proteinase system. Overexpression of TaPI4KIIγ revealed that it could enhance drought and salt stress tolerance during seed germination and seedling growth. A ubdkγ7 mutant, identified as an orthologue of TaPI4KIIγ in Arabidopsis, was sensitive to salt, polyethylene glycol (PEG), and abscisic acid (ABA), and overexpression of TaPI4KIIγ in the ubdkγ7 mutant compensated stress sensitivity. TaPI4KIIγ promoted root growth in Arabidopsis, suggesting that TaPI4KIIγ might enhance stress resistance by improving root growth. Overexpression of TaPI4KIIγ led to an altered expression level of stress-related genes and changes in several physiological traits that made the plants more tolerant to stress. The results provided evidence that overexpression of TaPI4KIIγ could improve drought and salt tolerance.
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影响因子：
7.4
作者：
Audenaert, K;De Meyer, GB;Höfte, MM
通讯作者：
Höfte, MM
影响因子：
4.8
作者：
JOHNSON, ES;MA, PCM;VARSHAVSKY, A
通讯作者：
VARSHAVSKY, A
影响因子：
4.9
作者：
Delage, Elise;Ruelland, Eric;Puyaubert, Juliette
通讯作者：
Puyaubert, Juliette
影响因子：
4.1
作者：
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通讯作者：
Boss, Wendy F.
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K