The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway.
The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway.
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DOI：
10.1186/1471-2180-10-281
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发表时间：
2010-11-09
期刊：
影响因子：
4.2
通讯作者：
Kiefer D
中科院分区：
文献类型：
--
作者：
Kreutel S;Kuhn A;Kiefer D
Rhodocista centenaria 是一种光养性 α-变形菌，表现出趋光行为，表现为在琼脂平板上红光照射下的菌落运动。与许多光养紫色细菌一样，R. centenaria 也拥有可溶性光活性黄色蛋白 (Pyp)。它以长融合蛋白的形式存在，命名为 Ppr，由三个结构域组成：Pyp 结构域、推定的胆碱结合结构域 (Bbd) 和组氨酸激酶结构域 (Pph)。 Ppr 蛋白参与聚酮化合物合成的调节，但目前尚不清楚其与趋光性和趋化性的关系。为了阐明 Ppr 和 Pph 在趋化网络中的可能作用，我们在体外和体内研究了与趋化蛋白的相互作用。进行基质辅助共洗脱实验来研究不同假定结合配偶体之间可能的通讯。发现 Ppr 蛋白的激酶结构域与趋化连接蛋白 CheW 相互作用。该复合物的形成显然依赖于 ATP。进一步的结果表明，Pph 组氨酸激酶结构域和 CheW 可能与趋化激酶 CheAY 形成复合物，表明 Ppr 在趋化信号通路中的作用。此外，当Ppr或Pph在大肠杆菌中表达时，细胞的趋化反应受到显着影响。 Rhodocista centenaria 的 Ppr 蛋白直接与趋化蛋白 CheW 相互作用。这表明 Ppr 蛋白除了在查耳酮合成中发挥作用外，还在趋化反应调节中发挥作用。
Rhodocista centenaria is a phototrophic α-proteobacterium exhibiting a phototactic behaviour visible as colony movement on agar plates directed to red light. As many phototrophic purple bacteria R. centenaria possesses a soluble photoactive yellow protein (Pyp). It exists as a long fusion protein, designated Ppr, consisting of three domains, the Pyp domain, a putative bilin binding domain (Bbd) and a histidine kinase domain (Pph). The Ppr protein is involved in the regulation of polyketide synthesis but it is still unclear, how this is connected to phototaxis and chemotaxis. To elucidate the possible role of Ppr and Pph in the chemotactic network we studied the interaction with chemotactic proteins in vitro as well as in vivo. Matrix-assisted coelution experiments were performed to study the possible communication of the different putative binding partners. The kinase domain of the Ppr protein was found to interact with the chemotactic linker protein CheW. The formation of this complex was clearly ATP-dependent. Further results indicated that the Pph histidine kinase domain and CheW may form a complex with the chemotactic kinase CheAY suggesting a role of Ppr in the chemotaxis signalling pathway. In addition, when Ppr or Pph were expressed in Escherichia coli, the chemotactic response of the cells was dramatically affected. The Ppr protein of Rhodocista centenaria directly interacts with the chemotactic protein CheW. This suggests a role of the Ppr protein in the regulation of the chemotactic response in addition to its role in chalcone synthesis.
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