Reprints Available Directly from the Publisher Photocopying Permitted by License Only Successful Xenogeneic Transplantation in Embryos" Induction of Tolerance by Extrathymic Chick Tissue Grafted into Quail
Reprints Available Directly from the Publisher Photocopying Permitted by License Only Successful Xenogeneic Transplantation in Embryos" Induction of Tolerance by Extrathymic Chick Tissue Grafted into Quail
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直接从出版商处获得重印 经许可证允许的复印 只有成功的胚胎异种移植” 移植到鹌鹑中的胸腺外雏鸡组织的耐受性诱导
DOI：
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发表时间：
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期刊：
影响因子：
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通讯作者：
N. L. Le Douarin
中科院分区：
文献类型：
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作者：
Claude Martin;H. Ohki‐Hamazaki;C. Corbel;M. Coltey;N. L. Le Douarin
在之前的实验中，我们已经证明，在E4胚胎期移植的肢体芽，在MHC错配的鸡胚胎之间，不仅在出生后耐受，而且在受体中诱导对表达供体MHC单倍型的细胞的分裂耐受状态：供体的皮肤移植物是永久耐受的，而供体型血细胞在MLR中产生宿主T细胞的增殖反应。如果进行同样的实验，以鹌鹑胚胎作为供体，小鸡作为受体，鹌鹑肢体的急性排斥反应在出生后的前两周开始，这表明在这些实验中诱导的外周型耐受只能在同种异体组合中获得，而不能在异种组合中获得。我们在这里报告了一个意想不到的结果，当鸡肢体芽在E4移植到鹌鹑中时，它是耐受的，并且像鸡的同种异体移植物一样，诱导成体皮肤移植物耐受而不改变MLR反应。另一种密切相关的鸟类——珍珠鸡科的珍珠鸡的移植物也得到了类似的结果。相比之下，涉及更遥远物种的异种组合（小鸡和鹌鹑作为受体，鸭科的鸭作为供体）导致两个受体的强烈和早期排斥。总体而言，鹌鹑在早期发育阶段就表现出比雏鸟更强的对周围抗原的耐受性。然而，在成年鹌鹑中，根据类似的时间模式，在任何方向（即，鹌鹑到小鸡或相反）进行的皮肤移植都会被拒绝。此外，两种物种的淋巴细胞对鹌鹑或雏鸡IL-2的反应同样良好。设想了几个假设来解释这些观察结果。这种类型的耐受性似乎与抗原负荷直接相关，因为雏鸟与鹌鹑翅膀嵌合体的抗原负荷大于鹌鹑与雏鸟嵌合体的抗原负荷。当将两个鹌鹑翅芽而不是一个翅芽移植到鸡身上时，观察到移植排斥反应的延迟，这一观点得到了支持。
In previous experiments, we have demonstrated that limb buds engrafted during embryonic life at E4, between MHC-mismatched chick embryos, are not only tolerated after birth, but induce in the recipient a state of split tolerance toward cells expressing the donor MHC haplotype: donor's skin grafts are permanently tolerated while a proliferative response of host's T cells is generated in MLR by donor-type blood cells. If the same experiment is performed, using quail embryo as a donor and chick as a recipient, acute rejection of the quail limb starts during the first two weeks after birth, thus suggesting that the peripheral type of tolerance induced in these experiments can be obtained only in allogeneic but not in xenogeneic combinations. We report here the unexpected result that when a chick limb bud is grafted into a quail at E4, it is tolerated and, like allogeneic grafts in chickens, induces adult skin-graft tolerance without modifying the MLR response. Similar results were obtained with grafts from another closely related species of bird, the guinea fowl from the Phasianidae family. In contrast, xenogeneic combinations involving more distant species (chick and quail as recipients and duck, an Anatidae, as donor) resulted in strong and early rejection from both recipients. As a whole, quails exhibit a greater ability than the chick to become tolerant to antigens presented peripherally from early developmental stages. In adult quails, however, skin grafts performed in either direction (i.e., quail to chick or the reverse) are rejected according to a similar temporal pattern. Moreover, lymphocytes of both species are able to respond equally well to quail or chick IL-2. Several hypotheses are envisaged to account for these observations. It seems likely that this type of tolerance is directly related to antigenic load because the load in chick to quail wing chimeras is larger than that in quail to chick chimeras. This view is supported by the protracted delay in graft rejection observed when two quail wing buds instead of one are grafted into chickens.
同种异体 II 类 MHC 分子的组织特异性表达既不引起组织排斥，也不引起同种异体反应性 T 细胞的克隆失活。
DOI：
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发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Miller,J;Daitch,L;Rath,S;Selsing,E
通讯作者：
Selsing,E
影响因子：
56.9
作者：
BALABAN, E;TEILLET, MA;LEDOUARIN, N
通讯作者：
LEDOUARIN, N