Establishment of an oral squamous cell carcinoma cell line expressing vascular endothelial growth factor a and its two receptors.
Establishment of an oral squamous cell carcinoma cell line expressing vascular endothelial growth factor a and its two receptors.
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表达血管内皮生长因子a及其两种受体的口腔鳞状细胞癌细胞系的建立。
DOI：
10.1016/j.jds.2022.04.018
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发表时间：
2022-10
影响因子：
3.5
通讯作者：
Kishimoto, Hiromitsu
中科院分区：
文献类型：
--
作者：
Araki-Maeda, Hanako;Kawabe, Mutsuki;Omori, Yuji;Yamanegi, Koji;Yoshida, Kazunari;Yoshikawa, Kyohei;Takaoka, Kazuki;Noguchi, Kazuma;Nakano, Yoshiro;Kishimoto, Hiromitsu
血管内皮生长因子受体（VEGFR）在口腔鳞状细胞癌（OSCC）中的表达通过自分泌和旁分泌信号通路促进肿瘤生长。vegf阳性的OSCC病例与浸润深度高、转移增加和预后差有关。在这项研究中，我们建立了一种共表达VEGF-A、VEGFR-1和VEGFR-2的OSCC细胞系，称为HCM-SqCC010细胞，并对其进行了分子和功能分析。采用免疫组化和免疫印迹法检测HCM-SqCC010细胞中VEGF-A、VEGFR-1和VEGFR-2的表达。通过实时荧光定量PCR验证VEGF-A、VEGFR-1、VEGFR-2在HCM-SqCC010细胞中的表达及抑制作用。我们对HCM-SqCC010细胞的分析显示，它们的增殖依赖于VEGF-A，选择性抑制VEGFR-1或VEGFR-2导致细胞生长下降。我们建立了表达VEGF-A、VEGFR-1和VEGFR-2的OSCC细胞系HCM-SqCC010。这种三阳性细胞系对VEGF-A的分子靶向药物没有影响，但对VEGFR抑制剂的反应确实显示出强烈的细胞生长抑制。因此，针对OSCC的新治疗策略应该包括VEGFR抑制剂。
Vascular endothelial growth factor receptor (VEGFR) expression in oral squamous cell carcinoma (OSCC) promotes tumor growth through both autocrine and paracrine signaling. VEGF-positive OSCC cases are associated with a high depth of invasion, increased metastasis, and poor prognosis. In this study we established and then molecularly and functionally analyzed an OSCC cell line that co-expresses VEGF-A, VEGFR-1, and VEGFR-2, termed HCM-SqCC010 cells. VEGF-A, VEGFR-1, and VEGFR-2 expression in HCM-SqCC010 cells were examined by immunohistochemistry and immunoblotting. Expression and inhibition of VEGF-A, VEGFR-1, and VEGFR-2 in HCM-SqCC010 cells were verified by quantitative real-time PCR. Our analysis of HCM-SqCC010 cells revealed that their proliferation depended on VEGF-A, and selective inhibition of VEGFR-1 or VEGFR-2 resulted in decreased cell growth. We established an OSCC cell line, HCM-SqCC010, that expresses VEGF-A, VEGFR-1, and VEGFR-2. This triple-positive cell line showed no effect from a molecular targeted drug toward VEGF-A, but it did show strong cell growth inhibition in response to a VEGFR inhibitor. Thus, new therapeutic strategies against OSCC should include a VEGFR inhibitor.
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影响因子：
4.3
作者：
Knizetova, Petra;Ehrmann, Jiri;Bartek, Jiri
通讯作者：
Bartek, Jiri
影响因子：
3.6
作者：
Mohamed, KM;Le, A;Messadi, DV
通讯作者：
Messadi, DV
影响因子：
21.3
作者：
Costa-Silva B;Aiello NM;Ocean AJ;Singh S;Zhang H;Thakur BK;Becker A;Hoshino A;Mark MT;Molina H;Xiang J;Zhang T;Theilen TM;García-Santos G;Williams C;Ararso Y;Huang Y;Rodrigues G;Shen TL;Labori KJ;Lothe IM;Kure EH;Hernandez J;Doussot A;Ebbesen SH;Grandgenett PM;Hollingsworth MA;Jain M;Mallya K;Batra SK;Jarnagin WR;Schwartz RE;Matei I;Peinado H;Stanger BZ;Bromberg J;Lyden D
通讯作者：
Lyden D
影响因子：
64.5
作者：
Lichtenberger, Beate M.;Tan, Poi Kiang;Sibilia, Maria
通讯作者：
Sibilia, Maria
影响因子：
1.2
作者：
Noguchi K;Kanda S;Yoshida K;Funaoka Y;Yamanegi K;Yoshikawa K;Takaoka K;Kishimoto H;Nakano Y
通讯作者：
Nakano Y