Changes to tetracyclines and tetracycline resistance genes in arable soils after single and multiple applications of manure containing tetracyclines
Changes to tetracyclines and tetracycline resistance genes in arable soils after single and multiple applications of manure containing tetracyclines
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单次和多次施用含四环素类有机肥后耕地土壤四环素类药物及四环素抗性基因的变化
DOI：
10.1007/s11356-017-0853-x
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发表时间：
2018-02
影响因子：
5.8
通讯作者：
Wang Meizhen
中科院分区：
文献类型：
--
作者：
Yan Huicong;Zhang Kun;Shentu Jiali;Shen Dongsheng;Li Na;Wang Meizhen
含有四环素（TCs）的粪便对土壤中抗生素抗性基因流行的影响仍然知之甚少。本研究将土霉素（OTC）、四环素（TC）和金四环素（CTC）三种不同的土霉素与未污染的粪肥混合施肥。单次施用0 μg kg−1、300 μg kg−1（TC和CTC）、700 μg kg−1（OTC）或每14天多次施用，连续140天。检测tet(A)、tet(L)、tet(M)、tet(Q) 4种四环素耐药基因（TRGs）。虽然单施处理4种TRGs的丰度在开始时迅速增加，但随着时间的推移，它们的丰度逐渐下降，并在第112天后显著低于重复处理。所有TCs的增加在第140天停止，但我们继续评估trg的长期积累。即使在施用含tc的肥料超过15个月后，大多数trg仍被检测到。重复施用含tc粪肥停止施肥后，TRGs丰度高于单施处理。因此，应注意在耕地土壤中反复施用含抗生素粪肥。
The influence of manure containing tetracyclines (TCs) on the prevalence of antibiotic resistance genes in soils remains poorly understood. Here, three different TCs (oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC)) were mixed respectively with unpolluted manure to fertilize arable soil. The soil received either a single application of 0 μg kg−1, 300 μg kg−1(TC and CTC), or 700 μg kg−1(OTC) or multiple applications every 14 days for 140 days. Four tetracycline resistance genes (TRGs), includingtet(A),tet(L),tet(M), andtet(Q), were monitored. Although the abundances of the four TRGs in the single application treatment initially increased rapidly, they decreased over time and were significantly lower than those of the repeated treatments after day 112. All additions of TCs stopped on day 140, but we continued to assess the long-term accumulation of TRGs. Most of the TRGs were detected even after the TC-containing manures had not been applied for more than 15 months. The abundance of the TRGs after ceasing fertilization with the TC-containing manures was higher in the repeated application treatments than in the single application treatments. Therefore, more attention should be paid to repeated applications of antibiotic-containing manure to arable soils.
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影响因子：
5.2
作者：
Fiedler, S.;Bender, J. K.;Werner, G.
通讯作者：
Werner, G.
影响因子：
3.3
作者：
Ng, LK;Martin, I;Mulvey, M
通讯作者：
Mulvey, M
影响因子：
4.4
作者：
Jechalke, Sven;Kopmann, Christoph;Smalla, Kornelia
通讯作者：
Smalla, Kornelia
DOI：
10.1073/pnas.1222743110
发表时间：
2013-02-26
影响因子：
11.1
作者：
Zhu, Yong-Guan;Johnson, Timothy A.;Tiedje, James M.
通讯作者：
Tiedje, James M.
影响因子：
2.2
作者：
Walsh, F.;Ingenfeld, A.;Duffy, B.
通讯作者：
Duffy, B.