NMR Reveals the Conformational Changes of Cytochrome C upon Interaction with Cardiolipin.
NMR Reveals the Conformational Changes of Cytochrome C upon Interaction with Cardiolipin.
复制标题
NMR 显示细胞色素 C 与心磷脂相互作用时的构象变化。
DOI：
10.3390/life11101031
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发表时间：
2021-09-30
期刊：
影响因子：
--
通讯作者：
Liu M
中科院分区：
文献类型：
--
作者：
Zhan J;Zhang G;Chai X;Zhu Q;Sun P;Jiang B;Zhou X;Zhang X;Liu M
细胞色素c（cyt c）与心磷脂（CL）相互作用引起的构象变化是细胞凋亡过程中的一个重要步骤，但其机制仍存在争议。为了全面阐明Cyt c与CL相互作用后的结构变化，避免蛋白质标记或突变可能导致的不可预测的别名，采用核磁共振技术，以蛋白质的三甲基化基团为天然探针，测定了不同CL含量下具有天然同位素丰度的纯化酵母iso-1 cyt c的构象.数据表明，细胞色素c有两个部分未折叠的构象时，与CL相互作用：一个与Fe-His 33协调和其他与五配位血红素。当CL含量较高时，Fe-His 33配位构象转变为五配位血红素构象。与CL相互作用后，cyt c的结构变得部分展开，更多暴露的血红素，表明cyt c喜欢线粒体中的高过氧化物酶活性状态，这反过来又使CL容易被氧化，并导致cyt c释放到细胞质中作为凋亡的触发器。
Conformational change of cytochrome c (cyt c) caused by interaction with cardiolipin (CL) is an important step during apoptosis, but the underlying mechanism is controversial. To comprehensively clarify the structural transformations of cyt c upon interaction with CL and avoid the unpredictable alias that might come from protein labeling or mutations, the conformation of purified yeast iso–1 cyt c with natural isotopic abundance in different contents of CL was measured by using NMR spectroscopy, in which the trimethylated group of the protein was used as a natural probe. The data demonstrate that cyt c has two partially unfolded conformations when interacted with CL: one with Fe–His33 coordination and the other with a penta–coordination heme. The Fe–His33 coordination conformation can be converted into a penta–coordination heme conformation in high content of CL. The structure of cyt c becomes partially unfolded with more exposed heme upon interaction with CL, suggesting that cyt c prefers a high peroxidase activity state in the mitochondria, which, in turn, makes CL easy to be oxidized, and causes the release of cyt c into the cytoplasm as a trigger in apoptosis.
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DOI：
10.1016/j.bbapap.2010.04.001
发表时间：
2010-07
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Biancalana M;Koide S
通讯作者：
Koide S
影响因子：
4.1
作者：
Milorey, Bridget;Schweitzer-Stenner, Reinhard;Malyshka, Dmitry
通讯作者：
Malyshka, Dmitry
影响因子：
2
作者：
Liu, M;Nicholson, JK;Tranter, GE
通讯作者：
Tranter, GE
影响因子：
7.4
作者：
Kagan, Valerian E.;Bayir, Huelya A.;Belikova, Natalia A.;Kapralov, Olexandr;Tyurina, Yulia Y.;Tyurin, Vladimir A.;Jiang, Jianfei;Stoyanovsky, Detcho A.;Wipf, Peter;Kochanek, Patrick M.;Greenberger, Joel S.;Pitt, Bruce;Shvedova, Anna A.;Borisenko, Grigory
通讯作者：
Borisenko, Grigory
影响因子：
15
作者：
Hong Y;Muenzner J;Grimm SK;Pletneva EV
通讯作者：
Pletneva EV