A fragment of engrailed regulatory DNA can mediate transvection of the white gene in Drosophila.
A fragment of engrailed regulatory DNA can mediate transvection of the white gene in Drosophila.
复制标题
一段被缠绕的调控 DNA 片段可以介导果蝇中白色基因的转染。
作者：
J. Kassis;E. P. VanSickle;Suzanne;Sensabaugh
我们在 P 元件转座子 CaSpeR 中发现了一段嵌入的调控 DNA 片段，该片段对连锁标记基因（白色）的表达具有不寻常的影响。通常，给定 CaSpeR 插入的纯合果蝇的眼睛比杂合子的颜色更深。然而，当转座子中包含特定的嵌入 DNA 片段时，纯合子的眼睛通常比杂合子的颜色更浅。因此，被缠绕的 DNA 似乎会导致纯合子中白色表达受到抑制。白色的抑制取决于基因组中两个转座子的接近程度——顺式（即在同一条染色体上）或反式（即在同源染色体上）。因此，被咬住的片段介导的现象与白色基因座的 zeste 基因介导的现象类似。然而，我们观察到的相互作用不需要zeste突变，也不受zeste突变的影响。我们认为，被嵌入的 DNA 含有一个或多个促进转座子之间相互作用的蛋白质的结合位点。这些位点的正常功能可能是介导 engrailed 的远距离顺式调控区域之间的相互作用，engrailed 是一个延伸超过 70 KB 的大基因座。
We have found a fragment of engrailed regulatory DNA that has an unusual effect on expression of a linked marker gene, white, in the P element transposon CaSpeR. Normally, flies homozygous for a given CaSpeR insertion have darker eyes than heterozygotes. However, when a particular engrailed DNA fragment is included in that transposon, homozygotes often have lighter eyes than heterozygotes. Thus, engrailed DNA appears to cause white expression to be repressed in homozygotes. The suppression of white is dependent on the proximity of the two transposons in the genome-either in cis (i.e., on the same chromosome) or in trans (i.e., on homologous chromosomes). Thus, the engrailed fragment is mediating a phenomenon similar to that mediated by the zeste gene at the white locus. However, the interactions we observe do not require, nor are influenced by, mutations of zeste. We suggest that the engrailed DNA contains one or more binding sites for a protein that facilitates interactions between transposons. The normal function of these sites may be to mediate interactions between distant cis-regulatory regions of engrailed, a large locus that extends over 70 kilobases.
影响因子：
3.3
作者：
H. Robertson;C. Preston;R. Phillis;D. Johnson-Schlitz;Wendy K. Benz;W. Engels
通讯作者：
H. Robertson;C. Preston;R. Phillis;D. Johnson-Schlitz;Wendy K. Benz;W. Engels