Gonococcal lipooligosaccharide sialylation prevents complement-dependent killing by immune sera
Gonococcal lipooligosaccharide sialylation prevents complement-dependent killing by immune sera
复制标题
淋球菌脂寡糖唾液酸化可防止免疫血清的补体依赖性杀伤
DOI：
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复制
发表时间：
1992
影响因子：
3.1
通讯作者：
E. C. Gotschlich
中科院分区：
文献类型：
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作者：
L M Wetzler;Kevin Barry;M. S. Blake;E. C. Gotschlich
先前的研究人员已经证明，当与5'- cmp - n -乙酰神经氨酸孵育时，唾液酸残基被添加到淋球菌低脂糖（LOS）的末端半乳糖部分。当这种体外唾液化发生时，淋球菌对正常人血清的杀菌活性产生耐药性。这被认为是由于添加的唾液酸残基阻断了存在于正常人血清中的杀菌抗los抗体的结合。我们扩展了这些研究，证明唾液化的淋球菌也对含有抗体的免疫血清的杀菌作用产生抗性，这些抗体可以识别除LOS外的外膜暴露成分。防止抗体与生物体结合并不是原因，因为与野生型生物体一样，唾液化生物体可以吸收与主要外膜蛋白（蛋白I）相同百分比的杀菌抗体。此外，淋球菌唾液化可阻止抗淋球菌抗血清的调理噬细胞作用。唾液酸对补体通路的负面影响可能是本研究结果的原因。
Previous investigators have demonstrated that a sialic acid residue is added to the terminal galactose moiety of gonococcal lipooligosaccharide (LOS) when incubated with 5'-CMP-N-acetylneuraminic acid. When this in vitro sialylation occurs, gonococci become resistant to the bactericidal activity of normal human serum. This is believed to result because the added sialic acid residue blocks the binding of bactericidal anti-LOS antibodies present in normal human serum. We extend these studies by demonstrating that sialylated gonococci also become resistant to the bactericidal effect of immune sera containing antibodies that recognize exposed components of the outer membrane besides LOS. Prevention of antibody binding to the organism was not the cause, since the same percentage of bactericidal antibodies to the major outer membrane protein, Protein I, can be absorbed with sialylated organisms as with wild-type organisms. In addition, gonococcal sialylation prevents opsonophagocytosis by antigonococcal antisera. The negative effect of sialic acid on the complement pathway might be the reason for the findings in this study.
DOI：
10.1093/cid/10.supplement_2.s287
发表时间：
1988
期刊：
Reviews of infectious diseases
影响因子：
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作者：
Griffiss,JM;Schneider,H;Mandrell,RE;Yamasaki,R;Jarvis,GA;Kim,JJ;Gibson,BW;Hamadeh,R;Apicella,MA
通讯作者：
Apicella,MA
两种相关细菌多糖上唾液酸的存在决定了初级免疫反应的部位以及补体耗竭对小鼠反应的影响。
DOI：
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发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Markham,RB;Nicholson-Weller,A;Schiffman,G;Kasper,DL
通讯作者：
Kasper,DL
淋病奈瑟菌的蛋白 I (Por) 作为免疫原：脂质体、蛋白体和缺乏封闭抗体。
DOI：
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发表时间：
1989
期刊：
Transactions of the Association of American Physicians
影响因子：
--
作者：
Wetzler,LM;Blake,MS;Gotschlich,EC
通讯作者：
Gotschlich,EC