miR-31-5p from placental and peripheral blood exosomes is a potential biomarker to diagnose preeclampsia.
miR-31-5p from placental and peripheral blood exosomes is a potential biomarker to diagnose preeclampsia.
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来自胎盘和外周血外泌体的 miR-31-5p 是诊断先兆子痫的潜在生物标志物
DOI：
10.1186/s41065-022-00250-z
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发表时间：
2022-09-19
期刊：
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
先兆子痫是一种病因不明的多系统疾病，是孕产妇和围产期发病率和死亡率的主要原因之一。识别敏感的非侵入性标志物可以帮助预防和改善预后。microRNAs（miRs）作为基因表达的负调控因子，与子痫前期的发生、发展密切相关。在此，我们研究了来自先兆子痫孕妇胎盘和外周血外泌体的DLK 1-Dio 3印迹miR簇与常规临床诊断指标之间的关系，并确定其作为非侵入性诊断标志物的潜力。从患有先兆子痫的孕妇的胎盘和外周血中提取外泌体。qPCR结果显示，子痫前期孕妇中miR-134、miR-31- 5 p、miR-655、miR-412、miR-539、miR-409和miR-496的表达水平显著低于健康对照组，其中miR-31- 5 p的表达差异最大。基因本体分析预测miR-31- 5 p负调控的基因主要富集在细胞实体、细胞过程和结合中;此外，京都基因百科全书和基因组通路分析表明，这些基因参与了促性腺激素释放激素受体通路和其他信号通路。相关性分析显示，miR-31- 5 p与子痫前期的收缩压、舒张压、乳酸脱氢酶、蛋白尿等临床指标呈显著负相关。我们认为，外泌体来源的miR-31- 5 p可以作为一种有效和敏感的生物标志物，以确定在孕妇先兆子痫的过程。
Preeclampsia, a multisystem disorder of unknown etiology, is one of the leading causes of maternal and perinatal morbidity and mortality. Identifying sensitive, noninvasive markers can aid its prevention and improve prognosis. microRNAs (miRs), which function as negative regulators of gene expression, are closely related to preeclampsia occurrence and development. Herein we investigated the relationship between the DLK1-Dio3 imprinted miR cluster derived from placental and peripheral blood exosomes of pregnant women with preeclampsia and routine clinical diagnostic indicators, and also determined its potential as a noninvasive diagnostic marker. Exosomes were extracted from the placenta and peripheral blood of pregnant women with preeclampsia. qPCR data indicated that the expression level of miRs, such as miR-134, miR-31-5p, miR-655, miR-412, miR-539, miR-409, and miR-496, in pregnant women with preeclampsia was significantly lower than that in healthy controls; miR-31-5p expression was the most different. Gene ontology analysis predicted that genes negatively regulated by miR-31-5p were mainly enriched in cellular entity, cellular process, and binding; moreover, Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that genes were involved in gonadotropin-releasing hormone receptor pathway and other signaling pathways. Correlation analysis revealed that miR-31-5p was significantly negatively correlated with clinical indicators of preeclampsia, such as systolic and diastolic pressure, lactate dehydrogenase, and proteinuria. We believe that exosome-derived miR-31-5p can serve as an effective and sensitive biomarker to determine the course of preeclampsia in pregnant women.
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影响因子：
8.5
作者：
Liu T;Jing F;Huang P;Geng Z;Xu J;Li J;Chen D;Zhu Y;Wang Z;Huang W;Chen C
通讯作者：
Chen C
DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
microRNA分析：方法和考虑因素。
DOI：
10.1038/nrg3198
发表时间：
2012-04-18
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
Pritchard CC;Cheng HH;Tewari M
通讯作者：
Tewari M
DOI：
10.1038/nrneph.2014.102
发表时间：
2014-08
期刊：
Nature reviews. Nephrology
影响因子：
--
作者：
Chaiworapongsa T;Chaemsaithong P;Yeo L;Romero R
通讯作者：
Romero R
影响因子：
3.7
作者：
Li L;Zhu D;Huang L;Zhang J;Bian Z;Chen X;Liu Y;Zhang CY;Zen K
通讯作者：
Zen K