Post separation adjustment of pH to enable the analysis of aminoglycoside antibiotics by microchip electrophoresis with amperometric detection
Post separation adjustment of pH to enable the analysis of aminoglycoside antibiotics by microchip electrophoresis with amperometric detection
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分离后调节 pH，以便通过微芯片电泳和安培检测分析氨基糖苷类抗生素
DOI：
10.1002/elps.201200309
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发表时间：
2012-11
期刊：
影响因子：
2.9
通讯作者：
丁永胜
中科院分区：
文献类型：
--
作者：
丁永胜
通过微芯片电泳和安培检测分析了五种氨基糖苷类抗生素（AG），即大观霉素、链霉素、阿米卡星、巴龙霉素和新霉素。虽然AGs的电泳分离是在酸性条件下进行的，但安培检测是在碱性条件下用电沉积Cu-Sn-Cr合金制成的电极进行的。为了实现这一目标，通过两个辅助通道将碱性溶液引入分离通道的末端，从而允许对每个分析操作使用不同的条件。除了建议的 pH 值调整外，使用 Cu-Sn-Cr 改性电极还可以延长电极的使用寿命。该合金被共电沉积在直径 25 μm 的铂丝上，然后集成到微芯片中并用作工作电极。此外，还研究了缓冲液的 pH 值和组成、分离电位、进样时间和滞留电位的影响，以优化 AG 的分离和检测。在最佳条件下，在4.9-316.8 μM范围内获得信号与浓度之间的线性关系，回归系数至少为0.99，LOD范围从阿米卡星的2.1 μM到壮观霉素的4.6 μM。通过分析加标牛奶样品中的五种氨基糖苷类抗生素，证明了该方法的适用性。结果表明，这种替代方法快速、灵敏且便携，能够分析牛奶样品中的氨基糖苷类抗生素。
Five aminoglycoside antibiotics (AGs), namely spectinomycin, streptomycin, amikacin, paromomycin, and neomycin, were analyzed by microchip electrophoresis with amperometric detection. Although the electrophoretic separation of AGs was carried out under acidic conditions, the amperometric detection was performed under alkaline conditions with an electrode fabricated by electrodeposition of an alloy of Cu‐Sn‐Cr. To achieve this, an alkaline solution was introduced into the end of the separation channel through two auxiliary channels, allowing the use of different conditions for each analytical operation. Along with the proposed pH adjustment, the use of electrodes modified with Cu‐Sn‐Cr enabled extending the lifetime of the electrodes. The alloy was co‐electrodeposited on a 25‐μm diameter platinum wire, which was then integrated in the microchip and used as working electrode. Furthermore, the effects of the pH and composition of buffer, separation potential, injection time, and detention potential were investigated in an effort to optimize both the separation and detection of AGs. Under the optimum conditions, linear relationships between the signal and the concentration were obtained in the 4.9–316.8 μM range, with regression coefficients of at least 0.99 and LODs ranging from 2.1 μM for amikacin to 4.6 μM for spectinomycin. Applicability of the method was demonstrated by analyzing five aminoglycoside antibiotics in spiked milk samples. The results showed that this alternative method is rapid, sensitive, and portable and enables the analysis of aminoglycoside antibiotics in milk sample.
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