Infectious in vitro transcripts from a cDNA clone of a Japanese gentian isolate of Sikte waterborne virus, which shows host-specific low-temperature-dependent replication

Infectious in vitro transcripts from a cDNA clone of a Japanese gentian isolate of Sikte waterborne virus, which shows host-specific low-temperature-dependent replication
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Sikte 水性病毒日本龙胆分离株的 cDNA 克隆的感染性体外转录本,显示宿主特异性低温依赖性复制

DOI:
10.1007/s00705-021-05074-2
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发表时间:
2021
影响因子:
2.7
通讯作者:
Mise Kazuyuki
Mise Kazuyuki
中科院分区:
医学4区
文献类型:
--
作者:
Fujisaki Koki;Tateda Chika;Abe Yoshiko;Dominguez John Jewish A.;Iwai Mari;Obara Kazue;Nakamura Taiki;Iwadate Yasuya;Kaido Masanori;Mise Kazuyuki

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已在几种作物中鉴定出桐状病毒,包括日本龙胆病毒A (GeVA)。本研究从日本龙胆草中分离到了另一种桐形病毒,即水媒病毒C1株(SWBV-C1)。虽然SWBV-C1和GeVA并不密切相关,但SWBV-C1和GeVA一样,在龙胆和拟南芥中表现出宿主特异性的低温依赖性复制。利用SWBV-C1基因组RNA全长cDNA克隆的体外转录本作为接种物证实了这些特性,表明鉴定的基因组RNA序列编码了SWBV-C1特征的病毒因子。
Tombusviruses have been identified in several crops, including gentian virus A (GeVA) in Japanese gentian. In this study, we isolated another tombusvirus, Sikte waterborne virus strain C1 (SWBV-C1), from Japanese gentian. Although SWBV-C1 and GeVA are not closely related, SWBV-C1, like GeVA, showed host-specific low-temperature-dependent replication in gentian and arabidopsis. The use ofin vitrotranscripts from full-length cDNA clones of SWBV-C1 genomic RNA as inocula confirmed these properties, indicating that the identified genomic RNA sequences encode viral factors responsible for the characteristic features of SWBV-C1.
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