Drosophila proboscis extension response and GCaMP imaging for assaying food appeal based on grittiness.
Drosophila proboscis extension response and GCaMP imaging for assaying food appeal based on grittiness.
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DOI：
10.1016/j.xpro.2022.101806
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发表时间：
2022-12-16
期刊：
影响因子：
--
通讯作者：
Montell, Craig
中科院分区：
文献类型：
--
作者：
Li, Qiaoran;Yang, Libo;Montell, Craig
果蝇感知食物的特征是由颗粒大小驱动的，包括光滑与否，通过装饰唇瓣的感受器的偏转，以及中- l神经元。我们描述了果蝇长鼻延伸反应实验的适应性，包括执行味觉测试和行为反应评分的步骤，以确定对不同大小颗粒食物的偏好。我们还描述了表达gcamp的果蝇的钙成像，以评估md-L神经元对不同程度的味觉感受器偏转的反应。有关使用和执行本协议的完整详情，请参阅。对苍蝇对光滑食物和砂砾食物的偏好进行评分的行为分析在苍蝇的唇瓣上精确压痕，通过砂砾食物模拟味觉毛发偏转Ca2+成像来评估md-L神经元对味觉毛发偏转的反应出版商注：开展任何实验方案都需要遵守当地机构的实验室安全和伦理指导方针。果蝇感知食物的特征是由颗粒大小驱动的，包括光滑与否，通过装饰唇瓣的感受器的偏转，以及中- l神经元。我们描述了果蝇长鼻延伸反应实验的适应性，包括执行味觉测试和行为反应评分的步骤，以确定对不同大小颗粒食物的偏好。我们还描述了表达gcamp的果蝇的钙成像，以评估md-L神经元对不同程度的味觉感受器偏转的反应。
Fruit flies sense the features of food that are driven by particle size, including smoothness versus grittiness, by deflection of sensilla decorating the labellum, and md-L neurons. We describe adaptation of the Drosophila proboscis extension response assay, including steps to perform the taste tests and score behavioral responses, to determine preferences to foods with different sized particles. We also describe calcium imaging in GCaMP-expressing flies to assess the responses of md-L neurons to different levels of taste sensilla deflection. For complete details on the use and execution of this protocol, please refer to. Behavioral assay to score a fly’s preference for smooth versus gritty food Precise indentation of the fly labellum to mimic taste hair deflection by gritty food Ca2+ imaging to assess responses of md-L neurons to deflection of taste hairs Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Fruit flies sense the features of food that are driven by particle size, including smoothness versus grittiness, by deflection of sensilla decorating the labellum, and md-L neurons. We describe adaptation of the Drosophila proboscis extension response assay, including steps to perform the taste tests and score behavioral responses, to determine preferences to foods with different sized particles. We also describe calcium imaging in GCaMP-expressing flies to assess the responses of md-L neurons to different levels of taste sensilla deflection.
DOI：
10.1016/j.cub.2021.02.007
发表时间：
2021-05-10
期刊：
Current biology : CB
影响因子：
--
作者：
Li Q;Montell C
通讯作者：
Montell C
影响因子：
16.2
作者：
Zhang YV;Aikin TJ;Li Z;Montell C
通讯作者：
Montell C
影响因子：
48
作者：
Schneider CA;Rasband WS;Eliceiri KW
通讯作者：
Eliceiri KW